The Effect of Composite Pig Islet-Human Endothelial Cell Grafts on the Instant Blood-Mediated Inflammatory Reaction

• Published in: Cell transplantation 2009-01, Vol.18 (1), p.31-38
• Main Authors: Kim, Hyoung-Il, Yu, Jae Eun, Lee, Song Yi, Sul, A. Young, Jang, Min Seok, Rashid, M. A., Park, Sang Gyu, Kim, Sang Jun, Park, Chung-Gyu, Kim, Jae Hyeon, Park, Kyong Soo
• Format: Article
• Language: English
• Subjects: Animals; Endothelial Cells - immunology; Endothelial Cells - transplantation; Graft Survival - immunology; Humans; Inflammation - blood; Inflammation - immunology; Islets of Langerhans - cytology; Islets of Langerhans - immunology; Islets of Langerhans Transplantation - immunology; Swine; Transplantation, Heterologous - immunology
• ISSN: 0963-6897; 1555-3892
• DOI: 10.3727/096368909788237113

Instant blood-mediated inflammatory reaction (IBMIR) causes rapid islet loss in portal vein islet transplantation. Endothelial cells are known to protect against complement-mediated lysis and activation of coagulation. We tested composite pig islet–human endothelial cell grafts as a strategy to overcome IBMIR. Porcine islets were cocultured with human endothelial cells in specially modified culture medium composed of M199 and M200 for 1–9 days. A positive control group, negative control group, and the endothelial cell-coated group were examined with an in vitro tubing loop assay using human blood. The endothelial cell-coated group was subdivided and analyzed by degree of surface coverage by endothelial cells (≤50% vs. >50%) or coculture time (