Metagenomic analysis of diarrheal stools in Kolkata, India, indicates the possibility of subclinical infection of Vibrio cholerae O1

We examined the stools of 23 patients in Kolkata, who were diagnosed as cholera patients because Vibrio cholerae O1 was detected from their stools by culturing methods, and further explored by metagenomic sequencing analysis. Subsequently, the presence of the gene encoding A subunit of cholera toxin...

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Bibliographic Details
Published in:Scientific reports 2022-11, Vol.12 (1), p.19473-13, Article 19473
Main Authors: Takahashi, Eizo, Motooka, Daisuke, Nakamura, Shota, Miyoshi, Shin-ichi, Chowdhury, Goutam, Mukhopadhyay, Asish K., Dutta, Shanta, Morita, Daichi, Iida, Tetsuya, Okamoto, Keinosuke
Format: Article
Language:English
Subjects:
631/158
631/326
692/4020
Asymptomatic Infections
Cholera
Cholera - diagnosis
Cholera - epidemiology
Cholera toxin
Cholera Toxin - genetics
Diarrhea
Diarrhea - epidemiology
Humanities and Social Sciences
Humans
India - epidemiology
Metagenomics
multidisciplinary
Science
Science (multidisciplinary)
Sequence analysis
Streptococcus infections
Subclinical infection
Toxins
Vibrio cholerae
Vibrio cholerae O1 - genetics
Waterborne diseases
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Summary:We examined the stools of 23 patients in Kolkata, who were diagnosed as cholera patients because Vibrio cholerae O1 was detected from their stools by culturing methods, and further explored by metagenomic sequencing analysis. Subsequently, the presence of the gene encoding A subunit of cholera toxin ( ctxA ) and the cholera toxin (CT) level in these stool samples were examined. ctxA was examined by both metagenomic sequencing analysis and polymerase chain reaction. In these examinations, two samples did not show positive in any of these tests. The metagenomic analysis showed that the genes for Streptococcus pneumoniae and Salmonella enterica were present in the stools of these two patients, respectively. Therefore, these two patients were not considered to have diarrhea due to V. cholerae infection. From these results, we predicted that some Kolkata residents harbor a small number of V. cholerae in their intestines as a form of subclinical infection with V. cholerae . Next, we analyzed the stool samples of 22 diarrhea patients from which V. cholerae was not isolated. The results showed that 3 of the patients seemed to have subclinical infection of V. cholerae based on the amount of the genes. These results indicated that subclinical infections with V. cholerae O1 occur in Kolkata.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-24167-9