Characterization of the Zebrafish Cell Landscape at Single-Cell Resolution

Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, we utilized Microwell-seq to analyze more than 250,000 single...

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Bibliographic Details
Published in:Frontiers in cell and developmental biology 2021-10, Vol.9, p.743421-743421
Main Authors: Jiang, Mengmeng, Xiao, Yanyu, E, Weigao, Ma, Lifeng, Wang, Jingjing, Chen, Haide, Gao, Ce, Liao, Yuan, Guo, Qile, Peng, Jinrong, Han, Xiaoping, Guo, Guoji
Format: Article
Language:English
Subjects:
Cell and Developmental Biology
cross-species analysis
regeneration
sequencing
single cell
zebrafish
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Summary:Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions and molecular dynamics during tissue regeneration in zebrafish remain poorly understood. Here, we utilized Microwell-seq to analyze more than 250,000 single cells covering major zebrafish cell types and constructed a systematic zebrafish cell landscape. We revealed single-cell compositions for 18 zebrafish tissue types covering both embryo and adult stages. Single-cell mapping of caudal fin regeneration revealed a unique characteristic of blastema population and key genetic regulation involved in zebrafish tissue repair. Overall, our single-cell datasets demonstrate the utility of zebrafish cell landscape resources in various fields of biological research.
ISSN:2296-634X
2296-634X
DOI:10.3389/fcell.2021.743421