A single-cell nanocoating of probiotics for enhanced amelioration of antibiotic-associated diarrhea

• Published in: Nature communications 2022-04, Vol.13 (1), p.2117-11, Article 2117
• Main Authors: Pan, Jiezhou, Gong, Guidong, Wang, Qin, Shang, Jiaojiao, He, Yunxiang, Catania, Chelsea, Birnbaum, Dan, Li, Yifei, Jia, Zhijun, Zhang, Yaoyao, Joshi, Neel S., Guo, Junling
• Format: Article
• Language: English
• Subjects: 14/19; 14/34; 14/63; 147/135; 147/143; 38/47; 631/326; 639/301/54; 64/86; Animals; Anti-Bacterial Agents - adverse effects; Antibiotics; Bacteria; Diarrhea; Diarrhea - chemically induced; Diarrhea - drug therapy; Digestive system; Ferric ions; Gastrointestinal system; Gastrointestinal tract; Gram-Negative Bacteria; Gram-Positive Bacteria; Homeostasis; Humanities and Social Sciences; Humans; Immunoregulation; Inflammation; Intestinal microflora; Levofloxacin; Levofloxacin - therapeutic use; Microbiota; Microorganisms; multidisciplinary; Pathogens; Probiotics; Probiotics - therapeutic use; Rats; Science; Science (multidisciplinary); Signs and symptoms; Tannic acid
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-022-29672-z

The gut microbiota represents a large community of microorganisms that play an important role in immune regulation and maintenance of homeostasis. Living bacteria receive increasing interest as potential therapeutics for gut disorders, because they inhibit the colonization of pathogens and positively regulate the composition of bacteria in gut. However, these treatments are often accompanied by antibiotic administration targeting pathogens. In these cases, the efficacy of therapeutic bacteria is compromised by their susceptibility to antibiotics. Here, we demonstrate that a single-cell coating composed of tannic acids and ferric ions, referred to as ‘nanoarmor’, can protect bacteria from the action of antibiotics. The nanoarmor protects both Gram-positive and Gram-negative bacteria against six clinically relevant antibiotics. The multiple interactions between the nanoarmor and antibiotic molecules allow the antibiotics to be effectively absorbed onto the nanoarmor. Armored probiotics have shown the ability to colonize inside the gastrointestinal tracts of levofloxacin-treated rats, which significantly reduced antibiotic-associated diarrhea (AAD) resulting from the levofloxacin-treatment and improved some of the pre-inflammatory symptoms caused by AAD. This nanoarmor strategy represents a robust platform to enhance the potency of therapeutic bacteria in the gastrointestinal tracts of patients receiving antibiotics and to avoid the negative effects of antibiotics in the gastrointestinal tract. Here, the authors develop a polyphenol-based single-cell coating that forms a nanoarmor on the surface of probiotics, showing that protects from a wide range of antibiotics and enhances probiotic action against antibiotic-mediated diarrhea.