Oncostatin M Contributes to Airway Epithelial Cell Dysfunction in Chronic Rhinosinusitis with Nasal Polyps

• Published in: International journal of molecular sciences 2023-04, Vol.24 (7), p.6094
• Main Authors: Carsuzaa, Florent, Bequignon, Emilie, Bartier, Sophie, Coste, André, Dufour, Xavier, Bainaud, Matthieu, Lecron, Jean Claude, Louis, Bruno, Tringali, Stéphane, Favot, Laure, Fieux, Maxime
• Format: Article
• Language: English
• Subjects: Cells, Cultured; Chronic Disease; ciliary beating efficiency; Cytokines; Efficiency; Electrical junctions; Epithelial cells; Epithelial Cells - metabolism; epithelial electric resistance; Epithelium; Evaluation; Humans; IL-6; Inflammation; Interleukin 6; Interleukins; Life Sciences; Monoclonal antibodies; nasal epithelium; Nasal Mucosa - metabolism; Nasal Polyps; Omalizumab; Oncostatin M; Oncostatin M - metabolism; Oncostatin M - pharmacology; OSM; Outdoor air quality; Pathogenesis; Polymerase chain reaction; Polyps; Rhinitis; Rhinosinusitis; RNA-directed DNA polymerase; Sinusitis; Sinusitis - metabolism; Tight junctions; Tight Junctions - metabolism; Western blotting; Wound healing; Zonula occludens-1 protein
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24076094

Chronic rhinosinusitis with nasal polyps (CRSwNP) is a typical type-2 inflammation involving several cytokines and is associated with epithelial cell dysfunction. Oncostatin M (OSM) (belonging to the interleukin(IL)-6 family) could be a key driver of epithelial barrier dysfunction. Therefore, we investigated the presence of OSM and IL-6 and the expression pattern of tight junctions (TJs) in the nasal tissue of CRSwNP patients and controls using reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) and Western blotting. Then, their potential role in the epithelial barrier was evaluated in vitro in 27 different primary cultures of human nasal epithelial cells (HNECs) by measuring TJ expression and transepithelial electric resistance (TEER) with or without OSM or IL-6 (1, 10, and 100 ng/mL). The effect on ciliary beating efficiency was evaluated by high-speed videomicroscopy and on repair mechanisms with a wound healing model with or without OSM. OSM and IL-6 were both overexpressed, and TJ (ZO-1 and occludin) expression was decreased in the nasal polyps compared to the control mucosa. OSM (100 ng/mL) but not IL-6 induced a significant decrease in TJ expression, TEER, and ciliary beating efficiency in HNECs. After 24 h, the wound repair rate was significantly higher in OSM-stimulated HNECs at 100 ng/mL. These results suggest that OSM could become a new target for monoclonal antibodies.