Protective Effects of Huo Xiang Zheng Qi Liquid on Clostridioides difficile Infection on C57BL/6 Mice

-associated disease (CDAD) is a major healthcare-associated infection. New treatment options for CDAD are needed. A traditional Chinese medicinal formula, Huo Xiang Zheng Qi (HXZQ), was chosen to test against CDAD in a mouse model. C57BL/6 mice were challenged with (ATCC 43255) orally; then received...

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Bibliographic Details
Published in:Microorganisms (Basel) 2024-08, Vol.12 (8), p.1602
Main Authors: Chen, Ming, Zhai, Lin, Schønning, Kristian, Alpízar-Alpízar, Warner, Larum, Ole, Andersen, Leif Percival, Holck, Susanne, Friis-Møller, Alice
Format: Article
Language:English
Subjects:
Animals
Antibacterial agents
Antibiotics
Body weight
C. difficile-associated disease
Clostridioides difficile
Diarrhea
Dilution
Drinking water
Ethanol
Feces
Fidaxomicin
Health aspects
Huo Xiang Zheng Qi
Infection
Infections
Metronidazole
Mice
Survival
Toxins
Traditional Chinese medicine
Vancomycin
Weight loss
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Summary:-associated disease (CDAD) is a major healthcare-associated infection. New treatment options for CDAD are needed. A traditional Chinese medicinal formula, Huo Xiang Zheng Qi (HXZQ), was chosen to test against CDAD in a mouse model. C57BL/6 mice were challenged with (ATCC 43255) orally; then received saline; vancomycin 25 mg/kg; or HXZQ in two different concentrations twice daily for 5 days. The animals' body weight; clinical signs; and survival rates were registered daily. Fecal pellets from each animal were taken for PCR analysis as a control of infection. 50% of the mice receiving saline died; 85.7% of the mice receiving vancomycin survived; 75% of the mice receiving HXZQ survived; and 87.5% of the mice receiving a 1:1 saline dilution of HXZQ survived. The HXZQ-treated groups were PCR positive with loads less than that of the untreated mice. The weight loss in the vancomycin plus HXZQ 1:1 treated group; the vancomycin-treated group; and the untreated group were 3.08%, 4.06%, and 9.62%, respectively. our results showed that HXZQ can protect mice from CDAD-related death as effectively as vancomycin and the combination of vancomycin and HXZQ may give even better protection.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms12081602