Glutathione during Post-Thaw Recovery Culture Can Mitigate Deleterious Impact of Vitrification on Bovine Oocytes

Vitrification of bovine oocytes can impair subsequent embryo development mostly due to elevated oxidative stress. This study was aimed at examining whether glutathione, a known antioxidant, can improve further embryo development when added to devitrified oocytes for a short recovery period. Bovine i...

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Bibliographic Details
Published in:Antioxidants 2022-12, Vol.12 (1), p.35
Main Authors: Olexiková, Lucia, Dujíčková, Linda, Makarevich, Alexander V, Bezdíček, Jiří, Sekaninová, Jana, Nesvadbová, Andrea, Chrenek, Peter
Format: Article
Language:English
Subjects:
Actin
Antioxidants
Apoptosis
Blastocysts
bovine oocyte
Catalase
Cell culture
Cryopreservation
Cytoskeleton
Design of experiments
Electron microscopy
Embryos
Enzymes
glutathione
Glutathione reductase
In vitro fertilization
Mitochondria
Nitrogen
Oocytes
Ovaries
Oxidative stress
Physiology
Potassium
Proteins
Sucrose
Vitrification
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Summary:Vitrification of bovine oocytes can impair subsequent embryo development mostly due to elevated oxidative stress. This study was aimed at examining whether glutathione, a known antioxidant, can improve further embryo development when added to devitrified oocytes for a short recovery period. Bovine in vitro matured oocytes were vitrified using an ultra-rapid cooling technique on electron microscopy grids. Following warming, the oocytes were incubated in the recovery medium containing glutathione (0, 1.5, or 5 mmol L ) for 3 h (post-warm recovery). Afterwards, the oocytes were lysed for measuring the total antioxidant capacity (TAC), activity of peroxidase, catalase and glutathione reductase, and ROS formation. The impact of vitrification on mitochondrial and lysosomal activities was also examined. Since glutathione, added at 5 mmol L , significantly increased the TAC of warmed oocytes, in the next set of experiments this dose was applied for post-warm recovery of oocytes used for IVF. Glutathione in the recovery culture did not change the total blastocyst rate, while increased the proportion of faster developing blastocysts (Day 6-7), reduced the apoptotic cell ratio and reversed the harmful impact of vitrification on the actin cytoskeleton. These results suggest that even a short recovery culture with antioxidant(s) can improve the development of bovine devitrified oocytes.
ISSN:2076-3921
2076-3921
DOI:10.3390/antiox12010035