Bile duct ligature in young rats: A revisited animal model for biliary atresia

Biliary atresia leads to cirrhosis in the vast majority of patients and constitutes the first cause of paediatric liver transplantation. Animal models allow us to understand the molecular basis and natural history of diseases. The aim of this study is to describe a surgically created animal model of...

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Bibliographic Details
Published in:European journal of histochemistry 2017-09, Vol.61 (3), p.2803-2803
Main Authors: Garrido, Matias, Escobar, Camila, Zamora, Constanza, Rejas, Carolina, Varas, Juan, Párraga, Mario, San Martin, Sebastian, Montedónico, Sandra
Format: Article
Language:English
Subjects:
animal models
Animals
Apelin
bile duct ligature
Bile Ducts - pathology
Bile Ducts - surgery
Biliary atresia
Biliary Atresia - pathology
Carrier Proteins - genetics
Carrier Proteins - metabolism
collagen
Collagen - genetics
Collagen - metabolism
Disease Models, Animal
Gene Expression Regulation
Intracellular Signaling Peptides and Proteins
Ligation
Liver - pathology
Rats
Rats, Sprague-Dawley
RNA, Messenger - genetics
RNA, Messenger - metabolism
Spleen - pathology
Technical Note
Tgfβ
Transforming Growth Factor beta1 - metabolism
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Summary:Biliary atresia leads to cirrhosis in the vast majority of patients and constitutes the first cause of paediatric liver transplantation. Animal models allow us to understand the molecular basis and natural history of diseases. The aim of this study is to describe a surgically created animal model of biliary atresia with emphasis in long-term liver function. Forty-two 3-week-old Sprague-Dawley rats were randomly divided into two groups: bile duct ligature (BDL) and control. The animals were sacrificed on the 2nd, 4th, and 6th postoperative weeks. Blood samples were collected for liver function analysis. The spleen to body weight ratio was determined. Histopathological examination of liver tissue was performed by hematoxylin-eosin and Sirius red staining. Collagen quantification was determined by using colorimetric digital image analysis and was expressed as a percentage of total liver tissue area. Quantitative real-time polymerase chain reaction was performed to analyse gene expression levels of transforming growth factor-β1 (Tgfb1) and apeline (Apln) genes. Statistical analysis was performed where P
ISSN:1121-760X
2038-8306
DOI:10.4081/ejh.2017.2803