Ante- and post-mortem cellular injury dynamics in hybrid poplar foliage as a function of phytotoxic O3 dose

After reaching phytotoxic levels during the last century, tropospheric ozone (O3) pollution is likely to remain a major concern in the coming decades. Despite similar injury processes, there is astounding interspecific-and sometimes intraspecific-foliar symptom variability, which may be related to s...

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Bibliographic Details
Published in:PloS one 2023-03, Vol.18 (3), p.e0282006-e0282006
Main Authors: Turc, Benjamin, Jolivet, Yves, Cabané, Mireille, Schaub, Marcus, Vollenweider, Pierre
Format: Article
Language:English
Subjects:
Alkaloids
Biology and Life Sciences
Cell Death
Cellular Biology
Cellular Senescence
Chlorophyll
Chloroplasts
Cytology
Dynamic structural analysis
Dynamics
Foliage
Hypersensitive response
Injuries
Leaves
Life Sciences
Mesophyll
Oxidative stress
Ozone
Physical Sciences
Physiological responses
Physiology
Poplar
Populus
Research and Analysis Methods
Risk assessment
Senescence
Temporal variations
Thylakoid membranes
Thylakoids
Toxins, Biological
Tropospheric ozone
VOCs
Volatile organic compounds
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Summary:After reaching phytotoxic levels during the last century, tropospheric ozone (O3) pollution is likely to remain a major concern in the coming decades. Despite similar injury processes, there is astounding interspecific-and sometimes intraspecific-foliar symptom variability, which may be related to spatial and temporal variation in injury dynamics. After characterizing the dynamics of physiological responses and O3 injury in the foliage of hybrid poplar in an earlier study, here we investigated the dynamics of changes in the cell structure occurring in the mesophyll as a function of O3 treatment, time, phytotoxic O3 dose (POD0), leaf developmental stage, and mesophyll layer. While the number of Hypersensitive Response-like (HR-like) lesions increased with higher O3 concentrations and POD0, especially in older leaves, most structural HR-like markers developed after cell death, independent of the experimental factors. The pace of degenerative Accelerated Cell Senescence (ACS) responses depended closely on the O3 concentration and POD0, in interaction with leaf age. Changes in total chlorophyll content, plastoglobuli and chloroplast shape pointed to thylakoid membranes in chloroplasts as being especially sensitive to O3 stress. Hence, our study demonstrates that early HR-like markers can provide reasonably specific, sensitive and reliable quantitative structural estimates of O3 stress for e.g. risk assessment studies, especially if they are associated with degenerative and thylakoid-related injury in chloroplasts from mesophyll.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0282006