Development of a Monoclonal Antibody Against Porcine CD163 SRCR5 Domain Which Partially Blocks Infection of PRRSV

Porcine reproductive and respiratory syndrome virus (PRRSV), which seriously endangers the world pig industry, invades host cells through receptor-mediated endocytosis involving clathrin. CD163 is an essential receptor for PRRSV during its infection of cells. The scavenger receptor cysteine-rich 5 (...

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Published in:Frontiers in veterinary science 2020-11, Vol.7, p.597843-597843
Main Authors: Zhang, Yujiao, Zhang, Kuan, Zheng, Hao, Liu, Changlong, Jiang, Yifeng, Du, Nannan, Li, Liwei, Li, Guoxin, Yu, Lingxue, Zhou, Yanjun, Tong, Wu, Zhao, Kuan, Tong, Guangzhi, Gao, Fei
Format: Article
Language:English
Subjects:
CD163
epitope
monoclonal antibody
PRRSV
SRCR5
Veterinary Science
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Summary:Porcine reproductive and respiratory syndrome virus (PRRSV), which seriously endangers the world pig industry, invades host cells through receptor-mediated endocytosis involving clathrin. CD163 is an essential receptor for PRRSV during its infection of cells. The scavenger receptor cysteine-rich 5 (SRCR5) domain of the CD163 molecule is necessary for PRRSV infection, and interacts with glycoproteins GP2a and GP4 of PRRSV, allowing the virus to infect the host cells. In this study, a monoclonal antibody (mAb) against the SRCR5-6 region of porcine CD163 was developed, and the target epitope of the mAb was determined as 497 TWGTVCDSDF 506 , which is directly adjacent to the ligand-binding pocket (LBP) domain (487-495aa) of CD163. Further study indicated that the mAb could partially block PRRSV infection of its target cells, pulmonary alveolar macrophages. The mAb developed in the study may provide a foundation of antiviral therapy for PRRSV.
ISSN:2297-1769
2297-1769
DOI:10.3389/fvets.2020.597843