BMP3 Affects Cortical and Trabecular Long Bone Development in Mice

• Published in: International journal of molecular sciences 2022-01, Vol.23 (2), p.785
• Main Authors: Banovac, Ivan, Grgurevic, Lovorka, Rumenovic, Viktorija, Vukicevic, Slobodan, Erjavec, Igor
• Format: Article
• Language: English
• Subjects: Age Factors; Alizarin; Animals; Biomarkers; Biomedical materials; Bmp3; Bone and Bones - metabolism; Bone marrow; Bone morphogenetic protein 3; Bone Morphogenetic Protein 3 - genetics; Bone Morphogenetic Protein 3 - metabolism; Bone morphogenetic proteins; Bones; Calcification, Physiologic; Cancellous bone; Cartilage; Computed tomography; Cortical bone; Cortical Bone - metabolism; Epiphyseal growth plate; Female; Follicles; Gene Expression; Growth factors; Growth plate; Growth Plate - growth & development; Growth Plate - metabolism; Immunohistochemistry; Kinases; Localization; Long bone; Male; Mice; Mice, Knockout; micro-CT; MicroRNAs; Mineralization; Osteocytes; Osteogenesis - genetics; Proteins; Regression analysis; Rodents; Sex Factors; Skeleton; Staining; Trends; X-Ray Microtomography
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23020785

Bone morphogenetic proteins (BMPs) have a major role in tissue development. BMP3 is synthesized in osteocytes and mature osteoblasts and has an antagonistic effect on other BMPs in bone tissue. The main aim of this study was to fully characterize cortical bone and trabecular bone of long bones in both male and female mice. To investigate the effect of from birth to maturity, we compared mice with wild-type littermates at the following stages of postnatal development: 1 day (P0), 2 weeks (P14), 8 weeks and 16 weeks of age. deletion was confirmed using X-gal staining in P0 animals. Cartilage and bone tissue were examined in P14 animals using Alcian Blue/Alizarin Red staining. Detailed long bone analysis was performed in 8-week-old and 16-week-old animals using micro-CT. The reporter signal was localized in bone tissue, hair follicles, and lungs. Bone mineralization at 2 weeks of age was increased in long bones of mice. deletion was shown to affect the skeleton until adulthood, where increased cortical and trabecular bone parameters were found in young and adult mice of both sexes, while delayed mineralization of the epiphyseal growth plate was found in adult mice.