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LncRNA lncLLM Facilitates Lipid Deposition by Promoting the Ubiquitination of MYH9 in Chicken LMH Cells
The liver plays an important role in regulating lipid metabolism in animals. This study investigated the function and mechanism of in liver lipid metabolism in hens at the peak of egg production. The effect of on intracellular lipid content in LMH cells was evaluated by qPCR, Oil Red O staining, and...
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Published in: | International journal of molecular sciences 2024-10, Vol.25 (19), p.10316 |
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Main Authors: | , , , , , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites |
Online Access: | Get full text |
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Summary: | The liver plays an important role in regulating lipid metabolism in animals. This study investigated the function and mechanism of
in liver lipid metabolism in hens at the peak of egg production. The effect of
on intracellular lipid content in LMH cells was evaluated by qPCR, Oil Red O staining, and detection of triglyceride (TG) and cholesterol (TC) content. The interaction between
and MYH9 was confirmed by RNA purification chromatin fractionation (CHIRP) and RNA immunoprecipitation (RIP) analysis. The results showed that
increased the intracellular content of TG and TC and promoted the expression of genes related to lipid synthesis. It was further found that
had a negative regulatory effect on the expression level of MYH9 protein in LMH cells. The intracellular TG and TC content of MYH9 knockdown cells increased, and the expression of genes related to lipid decomposition was significantly reduced. In addition, this study confirmed that the role of
is at least partly through mediating the ubiquitination of MYH9 protein to accelerate the degradation of MYH9 protein. This discovery provides a new molecular target for improving egg-laying performance in hens and treating fatty liver disease in humans. |
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ISSN: | 1422-0067 1661-6596 1422-0067 |
DOI: | 10.3390/ijms251910316 |