Comparison of the diagnostic accuracy of commercial NS1-based diagnostic tests for early dengue infection

• Published in: Virology journal 2010-12, Vol.7 (1), p.361-361, Article 361
• Main Authors: Osorio, Lyda, Ramirez, Meleny, Bonelo, Anilza, Villar, Luis A, Parra, Beatriz
• Format: Article
• Language: English
• Subjects: Acquisitions & mergers; Adolescent; Adult; Aged; Aged, 80 and over; Antibodies, Viral - blood; Child; Child, Preschool; Data collection; Dengue; Dengue - diagnosis; Diagnosis; Diagnostic Tests, Routine - methods; Disease; Encephalitis; Enzyme-linked immunosorbent assay; Female; Health aspects; Health facilities; Humans; Immunoassay - methods; Immunoglobulins; Infant; Infant, Newborn; Male; Methods; Middle Aged; Physiological aspects; Reagent Kits, Diagnostic; Reproducibility of Results; Sensitivity and Specificity; Studies; Tropical diseases; Viral Nonstructural Proteins - blood; Virology - methods; Young Adult
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422X-7-361

We compared the diagnostic accuracy and reproducibility of commercially available NS1-based dengue tests and explored factors influencing their sensitivities. Paired analysis of 310 samples previously characterized as positive (n = 218) and negative (n = 92) for viral isolation and/or RT-PCR and/or IgM seroconversion. Masked samples were tested by two observers with Platelia™ Dengue NS1 Ag, second generation Pan-E™ Dengue Early ELISA, SD Dengue NS1 Ag ELISA, Dengue NS1 Ag STRIP™, and SD BIOLINE™ Dengue Duo (NS1/IgM/IgG). SD BIOLINE™ NS1/IgM/IgG had the highest sensitivity (80.7% 95%CI 75-85.7) with likelihood ratios of 7.4 (95%CI 4.1-13.8) and 0.21 (95%CI 0.16-0.28). The ELISA-format tests showed comparable sensitivities; all below 75%. STRIP™ and SD NS1 had even lower sensitivities (