Identification of antibiotic resistance genes in  Escherichia coli from subclinical mastitis milk in dairy cows and goats, East Java Province

• Published in: Veterinární medicína 2024-02, Vol.69 (2), p.35-41
• Main Authors: Widianingrum, Desy Cahya, Silaban, Denada Grace, Fanata, Wahyu Indra Duwi, Khasanah, Himmatul
• Format: Article
• Language: English
• Subjects: Amides; Antibiotic resistance; Antibiotics; Bacteria; Bacterial diseases; California mastitis test; Cattle; Cow's milk; Dairy cattle; Dairy farming; Drug resistance; E coli; Escherichia coli; Farms; Genes; Goat's milk; Goats; Mastitis; Milk; Multidrug resistance; multiple drug resistant; Original Paper; Polymerase chain reaction; sulfhydryl variable (shv); temoneira enzyme (tem); Udder; zoonosis; β-Lactam antibiotics
• ISSN: 0375-8427; 1805-9392
• DOI: 10.17221/80/2023-VETMED

Antibiotics are still used to treat mastitis in dairy cows in Indonesia. This study aimed to analyse antibiotic resistance genes in  ( ) from subclinical mastitis milk in East Java Province, Indonesia. The samples consisted of subclinical mastitis milk from cows and goats. A total of 592-quarter cow's milk and 71 goat's milk samples from both halves of the udder were collected from 67 farms in Lumajang, Banyuwangi, Malang, Sidoarjo, Jember, Pasuruan, Probolinggo, and Mojokerto. Subclinical mastitis samples were screened using the California mastitis test (CMT). was identified by phenotypic and genotypic methods. was confirmed with a primer specific to the polymerase chain reaction (PCR) technique. Gene resistance of  was tested using the multiplex-PCR (mPCR) technique with primers encoding the genes temoneira enzyme ( ), oxacillinase ( ), sulfhydryl variable ( ), and cefotaximase-munich IV ( ). These genes were chosen because mastitis treatment generally uses oxacilline and β-lactam antibiotics. All data obtained were analysed descriptively. The results show that six isolates of  (46.15%) carried a single resistance gene ( or  ) and two isolates (33.33%) were confirmed as multiple drug-resistant organisms (MDROs) ( and ). The resistance genes were found in samples originating from Blitar, Banyuwangi, Lumajang, and Pasuruan Regencies. This research implies that antibiotic-resistance genes found in  on certain farms are dangerous and may allow gene transmission to other bacteria that make treatment for mastitis or other bacterial infections ineffective.