Autophagy modulation effect on homotypic transfer of intracellular components via tunneling nanotubes in mesenchymal stem cells

Recent studies have proved the role of autophagy in mesenchymal stem cell (MSCs) function and regenerative properties. How and by which mechanism autophagy modulation can affect the juxtacrine interaction of MSCs should be addressed. Here, the role of autophagy was investigated in the formation of t...

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Published in:Stem cell research & therapy 2024-07, Vol.15 (1), p.189-15, Article 189
Main Authors: Sadeghsoltani, Fatemeh, Avci, Çığır Biray, Hassanpour, Parisa, Haiaty, Sanya, Rahmati, Mohamad, Mota, Ali, Rahbarghazi, Reza, Nemati, Maryam, Mahdipour, Mahdi, Talebi, Mehdi, Takanlou, Leila Sabour, Takanlou, Maryam Sabour, Mehdizadeh, Amir
Format: Article
Language:English
Subjects:
Adenine - analogs & derivatives
Adenine - pharmacology
Animals
Apoptosis
Apoptosis - drug effects
Autophagy
Cell Membrane Structures
Cells, Cultured
Ethylenediaminetetraacetic acid
Fatty acids
Humans
Mesenchymal stem cells
Mesenchymal Stem Cells - cytology
Mesenchymal Stem Cells - metabolism
Metformin
Metformin - pharmacology
Mitochondria - metabolism
Mitochondrial donation
Nanotubes
Nanotubes - chemistry
Stem cells
Tunneling nanotubes
Wnt signaling pathway
Wnt Signaling Pathway - drug effects
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Summary:Recent studies have proved the role of autophagy in mesenchymal stem cell (MSCs) function and regenerative properties. How and by which mechanism autophagy modulation can affect the juxtacrine interaction of MSCs should be addressed. Here, the role of autophagy was investigated in the formation of tunneling nanotubes (TNTs) and homotypic mitochondrial donation. MSCs were incubated with 15 µM Metformin (Met) and/or 3 µM 3-methyladenine (3-MA) for 48 h. The formation of TNTs was assessed using bright-field and SEM images. The mitochondria density and ΔΨ values were monitored using flow cytometry analysis. Using RT-PCR and protein array, the close interaction and shared mediators between autophagy, apoptosis, and Wnt signaling pathways were also monitored. The total fatty acid profile was assessed using gas chromatography. Data indicated the increase of TNT length and number, along with other cell projections after the induction of autophagy while these features were blunted in 3-MA-treated MSCs (p 
ISSN:1757-6512
1757-6512
DOI:10.1186/s13287-024-03813-1