Bioinspired Polydopamine Coatings Facilitate Attachment of Antimicrobial Peptidomimetics with Broad-Spectrum Antibacterial Activity

• Published in: International journal of molecular sciences 2022-03, Vol.23 (6), p.2952
• Main Authors: Browne, Katrina, Kuppusamy, Rajesh, Chen, Renxun, Willcox, Mark D P, Walsh, William R, Black, David StC, Kumar, Naresh
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Anti-Infective Agents - pharmacology; Antibacterial activity; Antibacterial materials; Antibiotic resistance; Antibiotics; Antimicrobial agents; antimicrobial coatings; antimicrobial peptides; antimicrobial peptidomimetics; Bacteria; Biocompatibility; biofilm-mediated infections; Biofilms; Biomaterials; Biomedical materials; Chemical modification; Coated Materials, Biocompatible - chemistry; Coated Materials, Biocompatible - pharmacology; Coatings; Contact angle; Dopamine; Drug resistance; E coli; Erythrocytes; Escherichia coli; Gene expression; Gram-negative bacteria; hospital-acquired infections; Indoles; Infections; Medical equipment; Membranes; Metabolism; Morphology; Peptides; Peptidomimetics; Peptidomimetics - pharmacology; Photoelectron spectroscopy; Photoelectrons; Polymers; Pseudomonas aeruginosa; Scanning electron microscopy; Spectrum analysis; Staphylococcus aureus; Surface chemistry; Surgical implants
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23062952

The prevention and treatment of biofilm-mediated infections remains an unmet clinical need for medical devices. With the increasing prevalence of antibiotic-resistant infections, it is important that novel approaches are developed to prevent biofilms forming on implantable medical devices. This study presents a versatile and simple polydopamine surface coating technique for medical devices, using a new class of antibiotics-antimicrobial peptidomimetics. Their unique mechanism of action primes them for activity against antibiotic-resistant bacteria and makes them suitable for covalent attachment to medical devices. This study assesses the anti-biofilm activity of peptidomimetics, characterises the surface chemistry of peptidomimetic coatings, quantifies the antibacterial activity of coated surfaces and assesses the biocompatibility of these coated materials. X-ray photoelectron spectroscopy and water contact angle measurements were used to confirm the chemical modification of coated surfaces. The antibacterial activity of surfaces was quantified for , and , with all peptidomimetic coatings showing the complete eradication of on surfaces and variable activity for Gram-negative bacteria. Scanning electron microscopy confirmed the membrane disruption mechanism of peptidomimetic coatings against . Furthermore, peptidomimetic surfaces did not lyse red blood cells, which suggests these surfaces may be biocompatible with biological fluids such as blood. Overall, this study provides a simple and effective antibacterial coating strategy that can be applied to biomaterials to reduce biofilm-mediated infections.