Variation in the Microbiome, Trichothecenes, and Aflatoxins in Stored Wheat Grains in Wuhan, China

Contamination by fungal and bacterial species and their metabolites can affect grain quality and health of wheat consumers. In this study, sequence analyses of conserved DNA regions of fungi and bacteria combined with determination of trichothecenes and aflatoxins revealed the microbiome and mycotox...

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Bibliographic Details
Published in:Toxins 2018-04, Vol.10 (5), p.171
Main Authors: Yuan, Qing-Song, Yang, Peng, Wu, Ai-Bo, Zuo, Dong-Yun, He, Wei-Jie, Guo, Mao-Wei, Huang, Tao, Li, He-Ping, Liao, Yu-Cai
Format: Article
Language:English
Subjects:
Abundance
Aflatoxins
Conserved sequence
Contamination
Deoxynivalenol
Deoxyribonucleic acid
DNA
fungal diversity
Fungi
Fusarium
Fusarium graminearum
Genera
Grain
Metabolites
metagenomics
Microbiomes
Mycotoxins
Nivalenol
Nucleotide sequence
Prediction models
Relative abundance
Species
Species classification
Trichothecenes
Wheat
wheat storage
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Summary:Contamination by fungal and bacterial species and their metabolites can affect grain quality and health of wheat consumers. In this study, sequence analyses of conserved DNA regions of fungi and bacteria combined with determination of trichothecenes and aflatoxins revealed the microbiome and mycotoxins of wheat from different silo positions (top, middle, and bottom) and storage times (3, 6, 9, and 12 months). The fungal community in wheat on the first day of storage (T₀) included 105 classified species (81 genera) and 41 unclassified species. Four species had over 10% of the relative abundance: (12%), (27%), (12%), and (12%). Fungal diversity and relative abundance of in wheat from top silo positions were significantly lower than at other silo positions during storage. Nivalenol and deoxynivalenol in wheat were 13⁻34% higher in all positions at 3 months compared to T₀, and mycotoxins in wheat from middle and bottom positions at 6 to 12 months were 24⁻57% higher than at T₀. The relative abundance of toxigenic and aflatoxins were low at T₀ and during storage. This study provides information on implementation and design of fungus and mycotoxin management strategies as well as prediction models.
ISSN:2072-6651
2072-6651
DOI:10.3390/toxins10050171