CD90 is not constitutively expressed in functional innate lymphoid cells

Huge progress has been made in understanding the biology of innate lymphoid cells (ILC) by adopting several well-known concepts in T cell biology. As such, flow cytometry gating strategies and markers, such as CD90, have been applied to indentify ILC. Here, we report that most non-NK intestinal ILC...

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Bibliographic Details
Published in:Frontiers in immunology 2023-04, Vol.14, p.1113735
Main Authors: Schroeder, Jan-Hendrik, Beattie, Gordon, Lo, Jonathan W, Zabinski, Tomasz, Powell, Nick, Neves, Joana F, Jenner, Richard G, Lord, Graham M
Format: Article
Language:English
Subjects:
CD90
Colitis - metabolism
Cytokines - metabolism
DSS-colitis
Dysbiosis - metabolism
fecal microbial transplant (FMT)
Humans
Immunity, Innate
Immunology
innate lymphoid cell (ILC)
intestine
Lymphocytes - metabolism
Thy-1 Antigens - immunology
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Summary:Huge progress has been made in understanding the biology of innate lymphoid cells (ILC) by adopting several well-known concepts in T cell biology. As such, flow cytometry gating strategies and markers, such as CD90, have been applied to indentify ILC. Here, we report that most non-NK intestinal ILC have a high expression of CD90 as expected, but surprisingly a sub-population of cells exhibit low or even no expression of this marker. CD90-negative and CD90-low CD127 ILC were present amongst all ILC subsets in the gut. The frequency of CD90-negative and CD90-low CD127 ILC was dependent on stimulatory cues and enhanced by dysbiosis . CD90-negative and CD90-low CD127 ILC were a potential source of IL-13, IFNγ and IL-17A at steady state and upon dysbiosis- and dextran sulphate sodium-elicited colitis. Hence, this study reveals that, contrary to expectations, CD90 is not constitutively expressed by functional ILC in the gut.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2023.1113735