Chia seeds oil ameliorate chronic immobilization stress-induced neurodisturbance in rat brains via activation of the antioxidant/anti-inflammatory/antiapoptotic signaling pathways

• Published in: Scientific reports 2023-12, Vol.13 (1), p.22409-22409, Article 22409
• Main Authors: Khalifa, Norhan E., Noreldin, Ahmed E., Khafaga, Asmaa F., El-Beskawy, Mohamed, Khalifa, Eman, El-Far, Ali H., Fayed, Abdel-Hasseb A., Zakaria, Abdeldayem
• Format: Article
• Language: English
• Subjects: 631/378; 631/443; 631/45; 692/617; Animals; Anti-Inflammatory Agents - analysis; Antioxidants; Antioxidants - analysis; Apoptosis; Calbindin-D28K; Caspase 3; Corticosterone; Corticotropin-releasing hormone; Cyclooxygenase 2 - analysis; Cyclooxygenase-2; Cytokines; Gene expression; Humanities and Social Sciences; Humans; Immobilization; Inflammation; Interleukin 1; Interleukin 6; Linolenic acid; Mental disorders; Molecular Docking Simulation; multidisciplinary; Oils & fats; Rats; Science; Science (multidisciplinary); Seeds; Seeds - chemistry; Signal Transduction; Synaptophysin
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-49061-w

Chronic immobilization stress plays a key role in several neuropsychiatric disorders. This investigation assessed the possible ameliorative effect of chia seed oil (CSO) against the neurodisturbance-induced in rats by chronic immobilization. Rats were randomly allocated into control, CSO (1 ml/kg b.wt./orally), restrained (6 h/day), CSO pre-restraint, and CSO post-restraint for 60 days. Results revealed a significant reduction in serum corticosterone level, gene expression of corticotrophin-releasing factor, pro-inflammatory cytokines, and oxidative biomarkers in restrained rats treated with CSO. The histopathological findings revealed restoring necrosis and neuronal loss in CSO-treated-restraint rats. The immunohistochemical evaluation revealed a significant reduction in the immuno-expression of caspase-3, nuclear factor kappa B, interleukin-6, and cyclooxygenase-2 (COX-2), and an elevation of calbindin-28k and synaptophysin expression compared to non-treated restraint rats. The molecular docking showed the CSO high affinity for several target proteins, including caspase-3, COX-2, corticotropin-releasing hormone binding protein, corticotropin-releasing factor receptors 1 and 2, interleukin-1 receptor types 1 and 2, interleukin-6 receptor subunits alpha and beta. In conclusion, CSO emerges as a promising candidate against stress-induced brain disruptions by suppressing inflammatory/oxidative/apoptotic signaling pathways due to its numerous antioxidant and anti-inflammatory components, mainly α-linolenic acid. Future studies are necessary to evaluate the CSO therapeutic impacts in human neurodisturbances.