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Cryptosporidiosis in Humans with Reference to the First Case of Cryptosporidium hominis Infection in Turkey
Aim: Cryptosporidiosis is a worldwide zoonosis. Microscopic examinations may fail due to indistinctive morphological peculiarities of causative species. Hence, molecular diagnostics has become more important. Methods: Stool samples from 150 patients were examined using carbol-fuchsin stain to determ...
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Published in: | Haseki tıp bülteni 2017-09, Vol.55 (3), p.194-198 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Aim: Cryptosporidiosis is a worldwide zoonosis. Microscopic
examinations may fail due to indistinctive morphological peculiarities
of causative species. Hence, molecular diagnostics has become more
important.
Methods: Stool samples from 150 patients were examined using
carbol-fuchsin stain to determine Cryptosporidium spp. oocysts.
Combined nested polymerase chain reaction-restriction fragment
length polymorphism (PCR-RFLP) technique was used for establishing
different species in positive samples. The samples were also screened
for other parasites by wet-mount and zinc sulfate flotation methods.
Results: Microscopic examinations and molecular techniques revealed
0.67% (1/150) and 8.93% (5/56) positivity, respectively. Nested PCRRFLP
enabled the detection of Cryptosporidium hominis (C. hominis) in
one sample, while Cryptosporidium parvum (C. parvum) was detected
in four samples. With this study, C. hominis was reported from humans
for the first time in Turkey. Among infected ones, three of which were
children, four patients excreted C. parvum oocysts had gastroenteritis,
and a patient positive for C. hominis had gastroenteritis accompanied
by nausea and vomiting. No Giardia spp. and Entamoeba spp. were
detected in all infected individuals.
Conclusion: C. parvum cases outnumbered C. hominis cases,
suggesting a zoonotic transmission although infected individuals were
living in an urban area where animal husbandry was not allowed.
However, water-borne pathogen contamination in the city’s water
supply is considered a factor for transmission. |
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ISSN: | 1302-0072 2147-2688 |
DOI: | 10.4274/haseki.47966 |