Detection of several carbapenems resistant and virulence genes in classical and hyper-virulent strains of Klebsiella pneumoniae isolated from hospitalized neonates and adults in Khartoum

• Published in: BMC research notes 2020-07, Vol.13 (1), p.312-312, Article 312
• Main Authors: Albasha, Aalaa Mahgoub, Osman, Esraa Hassan, Abd-Alhalim, Saga, Alshaib, Elianz F, Al-Hassan, Leena, Altayb, Hisham N
• Format: Article
• Language: English
• Subjects: Abscesses; Adult; Adults; Antibiotics; Antimicrobial agents; Bacteremia; beta-Lactam Resistance - genetics; Carbapenems; Ceftazidime; Chloramphenicol; Ciprofloxacin; Cross Infection - microbiology; Deoxyribonucleic acid; DNA; Drug resistance; Epidemiology; Female; Genes; Genes, Bacterial - genetics; Genetic aspects; Gentamicin; Humans; hvKp; Imipenem; Infant, Newborn; K. pneumoniae; Klebsiella; Klebsiella Infections - microbiology; Klebsiella pneumoniae; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - isolation & purification; Klebsiella pneumoniae - pathogenicity; Laboratories; Male; MDR; Meningitis; Microbial drug resistance; Microbial Sensitivity Tests; Mortality; Multidrug resistance; Neonates; Nosocomial infection; Nosocomial infection and; Nosocomial infections; Pneumonia; Research Note; Sudan; Urinary tract; Varieties; Virulence; Virulence (Microbiology); Virulence Factors - genetics; Wound infection; XDR
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-020-05157-4

Klebsiella pneumoniae (K. pneumoniae) involves both community-acquired and nosocomial infections. It is responsible for a wide variety of infections, including infections of the urinary tract, pneumonia, bacteremia, meningitis, wound infection and purulent abscesses. We constructed this study to detect several carbapenems resistant and virulence genes in classical and hyper-virulent strains of K. pneumoniae isolated from hospitalized neonates and adults in Khartoum state. Seventy percent of the isolates were resistant to ceftazidime, 18(30%) to ciprofloxacin, 23(38.3%) to chloramphenicol, 24(40%) to gentamicin and 8% to imipenem, 35% were multidrug-resistant, and 7% extensively drug-resistant, all blood isolates (n = 14) were resistant to ceftazidime. entB was the most predominant virulence gene (93.3%), followed by mrkD (78.3%), kfu (60%), K2 (51.7%), magA (18.3%) and rmpA (5%). bla was the most predominant carbapenem-resistant gene (68.3%), followed by bla (10%), bla (8.3%), and bla (3.3%). Eight hyper-virulent strains were positive for bla and two for bla genes.