Methanotrophy Alleviates Nitrogen Constraint of Carbon Turnover by Rice Root-Associated Microbiomes

• Published in: Frontiers in microbiology 2022-05, Vol.13, p.885087-885087
• Main Authors: Cao, Weiwei, Cai, Yuanfeng, Bao, Zhihua, Wang, Shuwei, Yan, Xiaoyuan, Jia, Zhongjun
• Format: Article
• Language: English
• Subjects: carbon and nitrogen flow; methane; methanotrophs; Microbiology; nitrogen fixation; stable isotope probing
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2022.885087

The bioavailability of nitrogen constrains primary productivity, and ecosystem stoichiometry implies stimulation of N fixation in association with carbon sequestration in hotspots such as paddy soils. In this study, we show that N fixation was triggered by methane oxidation and the methanotrophs serve as microbial engines driving the turnover of carbon and nitrogen in rice roots. N -stable isotope probing showed that N -fixing activity was stimulated 160-fold by CH oxidation from 0.27 to 43.3 μmol N g dry weight root biomass, and approximately 42.5% of the fixed N existed in the form of N-NH through microbial mineralization. Nitrate amendment almost completely abolished N fixation. Ecophysiology flux measurement indicated that methane oxidation-induced N fixation contributed only 1.9% of total nitrogen, whereas methanotrophy-primed mineralization accounted for 21.7% of total nitrogen to facilitate root carbon turnover. DNA-based stable isotope probing further indicated that gammaproteobacterial s-like methanotrophs dominated N fixation in CH -consuming roots, whereas nitrate addition resulted in the shift of the active population to alphaproteobacterial -like methanotrophs. Co-occurring pattern analysis of active microbial community further suggested that a number of keystone taxa could have played a major role in nitrogen acquisition through root decomposition and N fixation to facilitate nutrient cycling while maintaining soil productivity. This study thus highlights the importance of root-associated methanotrophs as both biofilters of greenhouse gas methane and microbial engines of bioavailable nitrogen for rice growth.