Proteomic Analysis for Finding Serum Pathogenic Factors and Potential Biomarkers in Multiple Myeloma

Background： Multiple myeloma （MM） is a malignant tumor, which takes the second place in malignant blood disease. The clinical syrnptoms are complicated that make more difficult to diagnose and therapy. Lots of researches locus on the proteins about MM in order to solve those problems. We used proteo...

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Bibliographic Details
Published in:Chinese medical journal 2015-04, Vol.128 (8), p.1108-1113
Main Authors: Zhang, Hong-Tao, Tian, En-Bing, Chen, Yu-Ling, Deng, Hai-Teng, Wang, Qing-Tao
Format: Article
Language:English
Subjects:
Analysis
Biological markers
Biomarkers
Biomarkers - blood
Biomarkers, Tumor - blood
Bone diseases
Cancer
Chromatography
Diagnosis
Glycoproteins
Humans
Identification
Insulin-like growth factors
Integrin Alpha-11
Isoform-1 of Multimerin-1
Liquid Chromatography-tandem Mass Spectrometry
Multiple Myeloma
Peptide Ligand Library Bead
Ions
Laboratories
Lasers
LC-MS
Life sciences
Ligands
Mass spectrometry
Multiple myeloma
Multiple Myeloma - blood
Original
Peptide Library
Peptides
Proteins
Proteomics
Proteomics - methods
Scientific imaging
Tandem Mass Spectrometry
Western印迹
多发性骨髓瘤
患者
生物标志物
致病因素
蛋白质组学
血清蛋白
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Summary:Background： Multiple myeloma （MM） is a malignant tumor, which takes the second place in malignant blood disease. The clinical syrnptoms are complicated that make more difficult to diagnose and therapy. Lots of researches locus on the proteins about MM in order to solve those problems. We used proteomic methods to find potential biomarkers in MM patients. Methods： We applied the peptide ligand library beads （PLLBs） to deplete high abundance proteins in serum for finding potential pathogenic factors and biomarkers of MM. Using 1D-Gel-liquid chromatography-tandem mass spectrometry （LC-MS/MS）, we identified 789 and 849 unique serum proteins in MM patients and in healthy controls, respectively. Results： Twenty-two proteins were found differentially expressed between the two groups including sernm anayloid A protein, vitamin D-binding protein isoform-1 precursor, plasma kallikrein, and apolipoprotein A-I. Changes of integrin alpha-11 and isoform-1 of multimerin-l were validated with Western blotting. The linkage of the differentially expressed proteins and the pathogenesis pathways of MM were discussed, Conclusions： PLLB combined with 1D-gel-LC-MS/MS analysis is an efficient method to identity differentially expressed proteins in serum from patients with MM.
ISSN:0366-6999
2542-5641
DOI:10.4103/0366-6999.155112