Distribution characteristics of bioaerosols inside pig houses and the respiratory tract of pigs

• Published in: Ecotoxicology and environmental safety 2021-04, Vol.212, p.112006, Article 112006
• Main Authors: Tang, Qian, Huang, Kai, Liu, Junze, Jin, Xiaoming, Li, Chunmei
• Format: Article
• Language: English
• Subjects: Aerosols; Air Microbiology - standards; Air Pollutants - analysis; Animals; Bacteria - isolation & purification; China; Dust; Environmental Monitoring - methods; Humans; Particulate matter; Particulate Matter - analysis; Pig house; Respiratory System - microbiology; Respiratory tract; RNA, Ribosomal, 16S; Seasons; Swine; Temperature
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2021.112006

Particulate matter (PM) is a carrier of many substances. Microorganisms are vital constituents contained in PM, and their varieties and concentrations are closely connected to human health and animal production. This study aimed to investigate the distribution characteristics of bioaerosols inside a pig house and in the respiratory tract of pigs. Environmental indices inside a nursery pig house were monitored in winter, including temperature, relative humidity, total suspended particulate (TSP), PM10, PM2.5, NH3, CO2, CO and NO. The concentrations of airborne culturable bacteria, fungi and Escherichia coli were detected. Then, 16S rRNA sequencing technology was applied to identify different-sized bioaerosols and bacteria in the respiratory tract of piglets. The results showed that the concentration of airborne culturable bacteria inside the pig house was significantly higher than that outside, and no significant difference was found among culturable fungi and Escherichia coli. The 16S rRNA results showed that the bacterial aerosols presented high similarity to the bacteria in the respiratory tract of piglets. The airborne bacterial aerosols within the size range of 1.1–3.3 µm showed high similarity to the bacteria in the lower respiratory tract (bronchus and lung) of piglets. In addition, four potential pathogenic bacterial genera (Escherichia-Shigella, Streptococcus, Acinetobacter, Pseudomonas) were identified both in the bacterial aerosols and the respiratory tract of piglets. These results will provide a significant scientific basis for exploring the potential risk of aerosols from animal houses to human and animal health. [Display omitted] •Bacterial aerosol concentration inside pig house was higher than outside.•Four pathogenic bacterial genera were identified in bioaersols and respiratory of pigs.•Microorganisms colonizing respiratory of pigs mainly source from the bioaerosls.