O-5 INTEGRATED ANALYSIS OF ARCHAEAL, FUNGAL AND PROTOZOAN GUT TRANSCRIPTOME IN METABOLIC ASSOCIATED FATTY LIVER DISEASE (MAFLD) IN ARGENTINA

• Published in: Annals of hepatology 2023-03, Vol.28, p.101015, Article 101015
• Main Authors: Suárez, Bárbara, Mascardi, María Florencia, Mazzini, Flavia Noelia, Vera, M. Ruda, Marciano, Sebastián, Casciato, Paola, Narvaez, Adrián, Haddad, Leila, Anders, Margarita, Orozco, Federico, Tamaroff, Ana Jesica, Cook, Frank, Gounarides, John, Gutt, Susana, Gadano, Adrián, dez García, Celia Mén, Marro, Martin L., Steinhardt, Alberto Penas, Trinks, Julieta
• Format: Article
• Language: English
• ISSN: 1665-2681; 2659-5982
• DOI: 10.1016/j.aohep.2023.101015

Fungi, archaea and protozoa are the least known members of the gut microbiome, but they could represent a niche for biomarkers discovery to risk-stratify MAFLD patients. This study aimed to identify gut metatranscriptomic signatures in MAFLD patients from Argentina. Stool samples, diet, demographic and clinical data were obtained from 33 biopsy-proven MAFLD patients (12 simple steatosis (SS) / 21 steatohepatitis (SH)) and 19 healthy volunteers (HV). PNPLA3 rs738409 SNP was genotyped. RNA-seq was performed in NovaSeq6000®. Data were analyzed with Maaslin2-v1.2.0, bioBakery-v1.8 and DESeq2-v4.1. BMI was higher among MAFLD patients than in HV (q=4.49 × 10−6). The risk GG genotype of PNPLA3-SNP was more prevalent among SH (q=0.0198). In MAFLD patients and in subjects with the GG genotype, differentially expressed genes (DEGs) of fungi, such as Fusarium proliferatum and Candida sorbophila, were up-regulated (q