Evaluation of two morphometric methods of bone loss percentages caused by periodontitis in rats in different locations

The present study evaluated morphometrically bone loss percentages in experimental periodontitis in rats, comparing different locations (lingual mandible, palatal maxilla and buccal maxilla) and two evaluation methods (distance and area methods). Ligatures were placed around the maxillary right seco...

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Bibliographic Details
Published in:Journal of applied oral science 2010-10, Vol.18 (5), p.493-497
Main Authors: Souza, Daniela Martins de, Prado, Fernanda de Almeida, Prado, Marcela de Almeida, Rocha, Rosilene Fernandes da, Carvalho, Yasmin Rodarte de
Format: Article
Language:English
Subjects:
Alveolar Bone Loss - etiology
Alveolar Bone Loss - pathology
Animals
DENTISTRY, ORAL SURGERY & MEDICINE
Disease Models, Animal
Experimental studies
Female
Ligation
Methodology
Original
Periodontal diseases
Periodontal Ligament
Periodontitis - chemically induced
Periodontitis - complications
Periodontitis - pathology
Rats
Rats, Wistar
Reference Values
Reproducibility of Results
Tooth Cervix - anatomy & histology
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Summary:The present study evaluated morphometrically bone loss percentages in experimental periodontitis in rats, comparing different locations (lingual mandible, palatal maxilla and buccal maxilla) and two evaluation methods (distance and area methods). Ligatures were placed around the maxillary right second molar and around the mandibular right first molar in 14 female Wistar rats. The contralateral molars served as intragroup controls. After 4 weeks, the rats were sacrificed and their mandible and maxilla were removed. The specimens were dissected and stained with methylene blue dye. Bone loss was evaluated by two different methods on the surfaces of the defleshed jaw. In the first method, the distance from the cementoenamel junction (CEJ) to the alveolar bone crest was measured in the roots of teeth associated with ligature. In the second method, the area of bone loss was determined using the alveolar tissue bone, CEJ and the proximal region of roots associated with the ligature as reference. The data were converted to bone loss percentages caused by ligature: (ligated - unligated) x 100/ligated. When comparing the distance and area methods, no statistically significant difference was observed (p>0.05). Both methodologies indicated that the maxilla presented greater bone loss than the mandible and it was more accentuated on the buccal side than on the palatal side (p
ISSN:1678-7757
1678-7765
1678-7765
1678-7757
DOI:10.1590/s1678-77572010000500011