Lnc-PSMA8-1 activated by GEFT promotes rhabdomyosarcoma progression via upregulation of mTOR expression by sponging miR-144-3p

• Published in: BMC cancer 2024-01, Vol.24 (1), p.79-79, Article 79
• Main Authors: Meng, Lian, Shang, Hao, Liu, Qianqian, Li, Zhenzhen, Wang, Xiaomeng, Li, Qianru, Li, Feng, Zhao, Zhenguo, Liu, Chunxia
• Format: Article
• Language: English
• Subjects: Analysis; Apoptosis; Bioinformatics; Biotechnology; Care and treatment; Cell culture; Cell growth; Cell Line, Tumor; Cell lines; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; ceRNA; Development and progression; DNA microarrays; Families & family life; GEFT; Gene expression; Gene Expression Regulation, Neoplastic; Genetic aspects; Health aspects; Humans; Immunoprecipitation; Kinases; lnc-PSMA8-1; Lymph nodes; Medical prognosis; Metastases; Metastasis; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; miR-144-3p; miRNA; Molecular modelling; mRNA; mTOR; Musculoskeletal system; Non-coding RNA; Polymerase chain reaction; Prevention; Rhabdomyosarcoma; Rhabdomyosarcoma - genetics; Rhabdomyosarcoma - metabolism; Risk factors; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA-binding protein; Stem cells; TOR protein; TOR Serine-Threonine Kinases - genetics; TOR Serine-Threonine Kinases - metabolism; Tumorigenesis; Up-Regulation
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-023-11798-y

GEFT is a key regulator of tumorigenesis in rhabdomyosarcoma (RMS), and overexpression of GEFT is significantly correlated with distant metastasis, lymph node metastasis, and a poor prognosis, yet the underlying molecular mechanism is still poorly understood. This study aimed to investigate and validate the molecular mechanism of GEFT-activated lncRNAs in regulating mTOR expression to promote the progression of RMS. GEFT-regulated lncRNAs were identified through microarray analysis. The effects of GEFT-regulated lncRNAs on the proliferation, apoptosis, invasion, and migration of RMS cells were confirmed through cell functional experiments. The target miRNAs of GEFT-activated lncRNAs in the regulation of mTOR expression were predicted by bioinformatics analysis combined with quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The expression of lnc-PSMA8-1, miR-144-3p, and mTOR was measured by qRT-PCR in RMS tissue samples and cell lines. The regulatory mechanisms of the lnc-PSMA8-1-miR-144-3p-mTOR signaling axis were verified by RNA-binding protein immunoprecipitation (RIP), a luciferase reporter assay, qRT-PCR analysis, Western blot analysis, and cell functional experiments. The microarray-based analysis identified 31 differentially expressed lncRNAs (fold change > 2.0, P