Quantification of DNA by a Thermal-Durable Biosensor Modified with Conductive Poly(3,4-ethylenedioxythiophene)

The general clinical procedure for viral DNA detection or gene mutation diagnosis following polymerase chain reaction (PCR) often involves gel electrophoresis and DNA sequencing, which is usually time-consuming. In this study, we have proposed a facile strategy to construct a DNA biosensor, in which...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2018-10, Vol.18 (11), p.3684
Main Authors: Gu, Yesong, Tseng, Po-Yuan, Bi, Xiang, Yang, Jason H C
Format: Article
Language:English
Subjects:
Biosensing Techniques - methods
Bridged Bicyclo Compounds, Heterocyclic - chemistry
DNA - analysis
DNA biosensor
Electric Conductivity
Electrochemical Techniques
Electrodes
electron transfer mediate
Gold
gold nanoparticle
Metal Nanoparticles
Platinum - chemistry
poly(3,4-ethylenedioxythiophene)
Polymers - chemistry
Polystyrenes - chemistry
Temperature
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Summary:The general clinical procedure for viral DNA detection or gene mutation diagnosis following polymerase chain reaction (PCR) often involves gel electrophoresis and DNA sequencing, which is usually time-consuming. In this study, we have proposed a facile strategy to construct a DNA biosensor, in which the platinum electrode was modified with a dual-film of electrochemically synthesized poly(3,4-ethylenedioxythiophene) (PEDOT) resulting in immobilized gold nanoparticles, with the gold nanoparticles easily immobilized in a uniform distribution. The DNA probe labeled with a SH group was then assembled to the fabricated electrode and employed to capture the target DNA based on the complementary sequence. The hybridization efficiency was evaluated with differential pulse voltammetry (DPV) in the presence of daunorubicin hydrochloride. Our results demonstrated that the peak current in DPV exhibited a linear correlation the concentration of target DNA that was complementary to the probe DNA. Moreover, the electrode could be reused by heating denaturation and re-hybridization, which only brought slight signal decay. In addition, the addition of the oxidized form of nicotinamide adenine dinucleotide (NAD⁺) could dramatically enhance the sensitivity by more than 5.45-fold, and the limit-of-detection reached about 100 pM.
ISSN:1424-8220
1424-8220
DOI:10.3390/s18113684