Evaluation of P5CS and ProDH activity in Paulownia tomentosa (Steud.) as an indicator of oxidative changes induced by drought stress

The aim of the study was to investigate changes in proline metabolism in seedlings of tree species during drought stress. One month old seedlings were exposed to moisture conditions at various levels (irrigation at 100, 75, 50 and 25% of field capacity), and then the material (leaves and roots) was...

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Published in:PeerJ (San Francisco, CA) CA), 2024-01, Vol.12, p.e16697, Article e16697
Main Authors: Kijowska-Oberc, Joanna, Wawrzyniak, Mikołaj K, Ciszewska, Liliana, Ratajczak, Ewelina
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Biochemical markers
Biochemistry
Biosynthesis
Catabolism
Climate Change Biology
Drought
Drought stress
Droughts
Ecology
Environmental Impacts
Enzymes
Hydrogen peroxide
Leaves
Metabolism
Oxidative changes
P5CS
Paulownia tomentosa
Physiological aspects
Plant Science
ProDH
Proline dehydrogenase
Proline metabolism
Seedlings
Seeds
Soil moisture
Tree seedlings
Trees
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Summary:The aim of the study was to investigate changes in proline metabolism in seedlings of tree species during drought stress. One month old seedlings were exposed to moisture conditions at various levels (irrigation at 100, 75, 50 and 25% of field capacity), and then the material (leaves and roots) was collected three times at 10-day intervals. The activity of enzymes involved in proline metabolism was closely related to drought severity; however, proline content was not directly impacted. The activity of pyrroline-5-carboxylate synthetase (P5CS), which catalyzes proline biosynthesis, increased in response to hydrogen peroxide accumulation, which was correlated with soil moisture. In contrast, the activity of proline dehydrogenase (ProDH), which catalyzes proline catabolism, decreased. Compared to proline, the activity of these enzymes may be a more reliable biochemical marker of stress-induced oxidative changes. The content of proline is dependent on numerous additional factors, , its degradation is an important alternative energy source. Moreover, we noted tissue-specific differences in this species, in which roots appeared to be proline biosynthesis sites and leaves appeared to be proline catabolism sites. Further research is needed to examine a broader view of proline metabolism as a cycle regulated by multiple mechanisms and differences between species.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.16697