Coagulase-positive methicillin-resistant Staphylococcus aureus circulating in clinical mastitic goats in Bangladesh

• Published in: Veterinary World 2020-07, Vol.13 (7), p.1303-1310
• Main Authors: Rana, Eaftekhar Ahmed, Das, Tridip, Dutta, Avijit, Rahman, Mizanur, Bostami, Mohammad Bayazid, Akter, Nasima, Barua, Himel
• Format: Article
• Language: English
• Subjects: Antimicrobial agents; Antimicrobial resistance; Body temperature; California mastitis test; clinical mastitis; Coagulase; coagulase-positive staphylococcus aureus; goat; Goats; Health aspects; Mammary gland; Mastitis; MecA protein; Methicillin; methicillin-resistant staphylococcus aureus; Microbial drug resistance; Milk; Multidrug resistance; Oxacillin; Police officers; Polymerase chain reaction; Staphylococcus; Staphylococcus aureus; Staphylococcus aureus infections; Staphylococcus infections; Tetracyclines; Udder
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2020.1303-1310

is argued as one of the principal organisms responsible for mammary gland infection in lactating goats, causing both clinical and subclinical mastitis. Being highly zoonotic potential, pathogen emergence of methicillin-resistant (MRSA) has a significant clinical impact on treatment and management of clinical mastitis. We conducted a cross-sectional study to investigate the prevalence of coagulase-positive (CoPS), antimicrobial resistance profile of spp., prevalence of MRSA, and association between different clinical parameters with CoPS. A total of 67 clinical mastitic goats were sampled based on clinical examination and California mastitis test. Standard bacteriological methods were performed to isolate and identify spp. CoPS were confirmed by gene using polymerase chain reaction (PCR). All staphylococcal isolates were further examined for antimicrobial susceptibility testing by disk diffusion method. MRSA was confirmed based on gene-based PCR. Here, 49 (73.13%; 95% confidence interval [CI], 61.41-82.35) samples were positive for spp., of which 17 (34.69%; 95% CI, 22.88-48.73) isolates were CoPS and rest of the isolates (32; 65.30%; 95% CI, 51.27-77.12) were identified as coagulase-negative spp. (coagulase-negative staphylococci [CNS]). Both, CoPS and CNS isolates displayed the highest resistance against tetracycline (76.47% and 75%, respectively) and oxacillin (70.58% and 68.75%, respectively). Notably, all staphylococcal isolates were multidrug-resistant (showed resistance to ≥3 classes of antimicrobials). gene was found in 6 (8.96%; 95% CI, 3.84-18.52) CoPS isolates indicating MRSA strains. Among different clinical parameters, presence of high body temperature (p