Study of Interactions between Amlodipine and Quercetin on Human Serum Albumin: Spectroscopic and Modeling Approaches

The aim of this study was to analyze the binding interactions between a common antihypertensive drug (amlodipine besylate-AML) and the widely distributed plant flavonoid quercetin (Q), in the presence of human serum albumin (HSA). Fluorescence analysis was implemented to investigate the effect of li...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2019-01, Vol.24 (3), p.487
Main Authors: Vaneková, Zuzana, Hubčík, Lukáš, Toca-Herrera, José Luis, Furtműller, Paul Georg, Valentová, Jindra, Mučaji, Pavel, Nagy, Milan
Format: Article
Language:English
Subjects:
Affinity
Albumin
amlodipine
Antihypertensives
Binding sites
Circular dichroism
Dichroism
Drugs
Flavonoids
Fluorescence
FT-IR
Human serum albumin
Hydrophobicity
Ligands
Metabolites
Molecular docking
molecular modeling
Pharmacokinetics
Proteins
Quercetin
Serum albumin
Spectrum analysis
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Summary:The aim of this study was to analyze the binding interactions between a common antihypertensive drug (amlodipine besylate-AML) and the widely distributed plant flavonoid quercetin (Q), in the presence of human serum albumin (HSA). Fluorescence analysis was implemented to investigate the effect of ligands on albumin intrinsic fluorescence and to define the binding and quenching properties. Further methods, such as circular dichroism and FT-IR, were used to obtain more details. The data show that both of these compounds bind to Sudlow's Site 1 on HSA and that there exists a competitive interaction between them. Q is able to displace AML from its binding site and the presence of AML makes it easier for Q to bind. AML binds with the lower affinity and if the binding site is already occupied by Q, it binds to the secondary binding site inside the same hydrophobic pocket of Sudlow's Site 1, with exactly the same affinity. Experimental data were complemented with molecular docking studies. The obtained results provide useful information about possible pharmacokinetic interactions upon simultaneous co-administration of the food/dietary supplement and the antihypertensive drug.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24030487