Proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells and ovarian surface epithelial cells

The limitation of current biomarker of early stage ovarian cancer and the anatomical location of ovarian (depths of the pelvic) make ovarian cancer difficult to be detected in early stage. Growing evidence shows exosomes as key information transmitters, it carried molecules, such as miRNAs, proteins...

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Bibliographic Details
Published in:Journal of ovarian research 2020-01, Vol.13 (1), p.9-9, Article 9
Main Authors: Cheng, Lin, Zhang, Kun, Qing, Yunan, Li, Dong, Cui, Manhua, Jin, Peng, Xu, Tianmin
Format: Article
Language:English
Subjects:
Acids
Analysis
Binding sites
Biological markers
Biomarkers
Cancer cells
Cancer diagnosis
Cell adhesion & migration
Collagen
Collagen (type V)
Diseases
DNA
Drug resistance
Epithelial cells
Exosome
Exosomes
Flow cytometry
Immunoglobulins
Lipase
Lipid composition
Lipidomics
Lipids
Lipoprotein lipase
MicroRNA
Ovarian cancer
Peptides
Protein composition
Proteins
Proteomics
Species
Telecommunications equipment
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Summary:The limitation of current biomarker of early stage ovarian cancer and the anatomical location of ovarian (depths of the pelvic) make ovarian cancer difficult to be detected in early stage. Growing evidence shows exosomes as key information transmitters, it carried molecules, such as miRNAs, proteins, lipids, double-stranded DNA have been reported as promising biomarkers in many diseases. However, little is known about the protein and lipid composition of ovarian cancer. Here, we report proteomic and lipidomic analysis of exosomes derived from ovarian cancer cells (SKOV-3) and ovarian surface epithelial cells (HOSEPiC). A total of 1433 proteins and 1227 lipid species were identified from two cell line derived exosomes. Several lipid species and proteins significantly differ in SKOV-3 derived exosomes compared to those from HOSEPiC. For example, we noted that ChE and ZyE species were in general more abundant in exosomes from SKOV-3 than from HOSEPiC; Collagen type V alpha 2 chain (COL5A2) and lipoprotein lipase (LPL) were significantly higher in SKOV-3 derived exosomes than HOSEpic (p 
ISSN:1757-2215
1757-2215
DOI:10.1186/s13048-020-0609-y