Microbe-metabolite interaction networks, antibiotic resistance, and in vitro reconstitution of the penile prosthesis biofilm support a paradigm shift from infection to colonization

To understand differences between asymptomatic colonized and infected states of indwelling medical devices, we sought to determine penile prosthesis biofilm composition, microbe-metabolite interaction networks, and association with clinical factors. Patients scheduled for penile prosthesis removal/r...

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Bibliographic Details
Published in:Scientific reports 2023-07, Vol.13 (1), p.11522-11522, Article 11522
Main Authors: Werneburg, Glenn T., Lundy, Scott D., Hettel, Daniel, Bajic, Petar, Gill, Bradley C., Adler, Ava, Mukherjee, Sromona D., Wood, Hadley M., Angermeier, Kenneth W., Shoskes, Daniel A., Miller, Aaron W.
Format: Article
Language:English
Subjects:
631/326/107
631/326/46
692/4025/2768/515
Antibiotic resistance
Asymptomatic
Biofilms
Bioreactors
Coronary artery disease
Diabetes mellitus
Heart diseases
Humanities and Social Sciences
Infections
Medical equipment
Metabolites
Metabolomics
Microorganisms
multidisciplinary
Next-generation sequencing
Penis
Polyurethane
Prostheses
Science
Science (multidisciplinary)
Silicones
Transplants & implants
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Summary:To understand differences between asymptomatic colonized and infected states of indwelling medical devices, we sought to determine penile prosthesis biofilm composition, microbe-metabolite interaction networks, and association with clinical factors. Patients scheduled for penile prosthesis removal/revision were included. Samples from swabbed devices and controls underwent next-generation sequencing, metabolomics, and culture-based assessments. Biofilm formation from device isolates was reconstituted in a continuous-flow stir tank bioreactor. 93% of 27 analyzed devices harbored demonstrable biofilm. Seven genera including Faecalibaculum and Jeotgalicoccus were more abundant in infected than uninfected device biofilms ( p  
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-38750-1