Arabidopsis AMINO ACID PERMEASE1 Contributes to Salt Stress-Induced Proline Uptake from Exogenous Sources

Stress-induced proline accumulation in plants is thought to result primarily from enhanced proline biosynthesis and decreased proline degradation. To identify regulatory components involved in proline transport, we screened for T-DNA mutants with enhanced tolerance to toxic levels of exogenous proli...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in plant science 2017-12, Vol.8, p.2182
Main Authors: Wang, Ting, Chen, Ying, Zhang, Min, Chen, Jiugeng, Liu, Jie, Han, Huiling, Hua, Xuejun
Format: Article
Language:English
Subjects:
amino acid permease
Arabidopsis thaliana
Plant Science
positional cloning
proline accumulation
proline transport
salt stress
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Stress-induced proline accumulation in plants is thought to result primarily from enhanced proline biosynthesis and decreased proline degradation. To identify regulatory components involved in proline transport, we screened for T-DNA mutants with enhanced tolerance to toxic levels of exogenous proline (45 mM). We isolated the ( ) mutant and map-based cloning identified as ( , At1g58360), which encodes a plasma membrane-localized amino acid permease. expression is induced by salt stress and abscisic acid, but not by proline. In mutants, a 19-nucleotide deletion in the coding region produced a premature stop codon. When grown on proline-containing medium, mutants accumulated significantly less proline than did the wild type. Under salt stress, proline uptake decreased significantly in mutants. By contrast, proline uptake increased significantly in the wild type. These results suggest that AAP1 functions in the increase of proline uptake during salt stress. In addition, proline uptake promotes salt tolerance in Arabidopsis seedlings. We conclude that plants can increase proline accumulation by AtAAP1-mediated proline uptake from exogenous source, which help to improve the salt tolerance of seedlings.
ISSN:1664-462X
1664-462X
DOI:10.3389/fpls.2017.02182