Optimization of Growth Conditions to Enhance PHA Production by Cupriavidus necator

The accumulation of polyhydroxyalkanoates (PHAs) by microorganisms usually occurs in response to environmental stress conditions. Therefore, it is advantageous to choose two-step cultivation. The first phase is aimed at maximizing biomass production, and only in the second phase, after setting the s...

Full description

Saved in:
Bibliographic Details
Published in:Fermentation (Basel) 2022-09, Vol.8 (9), p.451
Main Authors: Ronďošová, Soňa, Legerská, Barbora, Chmelová, Daniela, Ondrejovič, Miroslav, Miertuš, Stanislav
Format: Article
Language:English
Subjects:
Ammonium
Ammonium sulfate
Bacteria
Biomass
Carbon
Cupriavidus necator
Cupriavidus necator DSM 545
Environmental stress
Glucose
Growth conditions
Magnesium
Microorganisms
Nitrogen
optimization
poly(3-hydroxybutyrate)
Potassium
Propagation
response surface methodology
Sodium
Statistical analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The accumulation of polyhydroxyalkanoates (PHAs) by microorganisms usually occurs in response to environmental stress conditions. Therefore, it is advantageous to choose two-step cultivation. The first phase is aimed at maximizing biomass production, and only in the second phase, after setting the suitable conditions, PHA production starts. The aim of this work was to optimize the composition of the minimal propagation medium used for biomass production of Cupriavidus necator DSM 545 using the response surface methodology (RSM). Based on the results from the search for optimization limits, the glucose concentration, the ammonium sulfate concentration and the phosphate buffer molarity were chosen as independent variables. The optimal values were found as follows: the glucose concentration 10.8 g/L; the ammonium sulfate concentration 0.95 g/L; and the phosphate buffer molarity 60.2 mmol/L. The predicted biomass concentration was 4.54 g/L, and the verified value was at 4.84 g/L. Although this work was primarily focused on determining the optimal composition of the propagation medium, we also evaluated the optimal composition of the production medium and found that the optimal glucose concentration was 6.7 g/L; the ammonium sulfate concentration 0.60 g/L; and the phosphate buffer molarity 20 mmol/L. The predicted PHB yield was 54.7% (w/w) of dry biomass, and the verified value was 49.1%.
ISSN:2311-5637
2311-5637
DOI:10.3390/fermentation8090451