Persistence of Antibiotic-Resistant Escherichia coli Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions

is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant in wa...

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Bibliographic Details
Published in:Antibiotics (Basel) 2022-02, Vol.11 (2), p.202
Main Authors: Xie, Hui, Ogura, Yoshitoshi, Suzuki, Yoshihiro
Format: Article
Language:English
Subjects:
Aerobic conditions
Antibiotic resistance
antibiotic resistant
Antibiotics
Antimicrobial agents
Bacteria
Drug resistance
E coli
Escherichia coli
Minimum inhibitory concentration
Multidrug resistance
Municipal wastewater
Phylogeny
phylogroups
Staphylococcus infections
Survivability
Wastewater
Wastewater treatment
Water treatment
β Lactamase
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Summary:is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant in wastewater. In this study, the survival of in wastewater and the changes to the relationships between each phylogroup and the antibiotic-resistant profiles of isolates from wastewater were investigated under aerobic conditions for 14 days. The isolates were classified into the phylogroups A, B1, B2, and D or others by multiplex PCR. In addition, the susceptibility of the isolates to 11 antibiotics was assessed with the minimum inhibitory concentration (MIC) assay. While counts decreased in the wastewater with time under aerobic conditions, the prevalence of phylogroup B2 had increased to 73% on day 14. Furthermore, the MIC assay revealed that the abundance of antibiotic-resistant also increased on day 14. After batch-mixing the experiments under aerobic conditions, the surviving antibiotic-resistant included mainly multidrug-resistant and beta-lactamase-producing isolates belonging to phylogroup B2. These results suggest that the phylogroup B2 isolates that have acquired antibiotic resistance had a high survivability in the treated wastewater.
ISSN:2079-6382
2079-6382
DOI:10.3390/antibiotics11020202