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Persistence of Antibiotic-Resistant Escherichia coli Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions
is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant in wa...
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| Published in: | Antibiotics (Basel) 2022-02, Vol.11 (2), p.202 |
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| creator | Xie, Hui Ogura, Yoshitoshi Suzuki, Yoshihiro |
| description | is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant
is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant
in wastewater. In this study, the survival of
in wastewater and the changes to the relationships between each phylogroup and the antibiotic-resistant profiles of
isolates from wastewater were investigated under aerobic conditions for 14 days. The isolates were classified into the phylogroups A, B1, B2, and D or others by multiplex PCR. In addition, the susceptibility of the isolates to 11 antibiotics was assessed with the minimum inhibitory concentration (MIC) assay. While
counts decreased in the wastewater with time under aerobic conditions, the prevalence of phylogroup B2 had increased to 73% on day 14. Furthermore, the MIC assay revealed that the abundance of antibiotic-resistant
also increased on day 14. After batch-mixing the experiments under aerobic conditions, the surviving antibiotic-resistant
included mainly multidrug-resistant and beta-lactamase-producing isolates belonging to phylogroup B2. These results suggest that the phylogroup B2 isolates that have acquired antibiotic resistance had a high survivability in the treated wastewater. |
| doi_str_mv | 10.3390/antibiotics11020202 |
| format | article |
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is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant
in wastewater. In this study, the survival of
in wastewater and the changes to the relationships between each phylogroup and the antibiotic-resistant profiles of
isolates from wastewater were investigated under aerobic conditions for 14 days. The isolates were classified into the phylogroups A, B1, B2, and D or others by multiplex PCR. In addition, the susceptibility of the isolates to 11 antibiotics was assessed with the minimum inhibitory concentration (MIC) assay. While
counts decreased in the wastewater with time under aerobic conditions, the prevalence of phylogroup B2 had increased to 73% on day 14. Furthermore, the MIC assay revealed that the abundance of antibiotic-resistant
also increased on day 14. After batch-mixing the experiments under aerobic conditions, the surviving antibiotic-resistant
included mainly multidrug-resistant and beta-lactamase-producing isolates belonging to phylogroup B2. These results suggest that the phylogroup B2 isolates that have acquired antibiotic resistance had a high survivability in the treated wastewater.</description><identifier>ISSN: 2079-6382</identifier><identifier>EISSN: 2079-6382</identifier><identifier>DOI: 10.3390/antibiotics11020202</identifier><identifier>PMID: 35203805</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Aerobic conditions ; Antibiotic resistance ; antibiotic resistant ; Antibiotics ; Antimicrobial agents ; Bacteria ; Drug resistance ; E coli ; Escherichia coli ; Minimum inhibitory concentration ; Multidrug resistance ; Municipal wastewater ; Phylogeny ; phylogroups ; Staphylococcus infections ; Survivability ; Wastewater ; Wastewater treatment ; Water treatment ; β Lactamase</subject><ispartof>Antibiotics (Basel), 2022-02, Vol.11 (2), p.202</ispartof><rights>2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2022 by the authors. 2022</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c565t-51bb10e818392e209a478987b5aed985fbbae4c698f7d649c6bd55c33659f7f53</citedby><cites>FETCH-LOGICAL-c565t-51bb10e818392e209a478987b5aed985fbbae4c698f7d649c6bd55c33659f7f53</cites><orcidid>0000-0003-3550-9870 ; 0000-0002-1148-5379</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2632196513/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2632196513?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25730,27900,27901,36988,36989,44565,53765,53767,75095</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/35203805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xie, Hui</creatorcontrib><creatorcontrib>Ogura, Yoshitoshi</creatorcontrib><creatorcontrib>Suzuki, Yoshihiro</creatorcontrib><title>Persistence of Antibiotic-Resistant Escherichia coli Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions</title><title>Antibiotics (Basel)</title><addtitle>Antibiotics (Basel)</addtitle><description>is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant
is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant
in wastewater. In this study, the survival of
in wastewater and the changes to the relationships between each phylogroup and the antibiotic-resistant profiles of
isolates from wastewater were investigated under aerobic conditions for 14 days. The isolates were classified into the phylogroups A, B1, B2, and D or others by multiplex PCR. In addition, the susceptibility of the isolates to 11 antibiotics was assessed with the minimum inhibitory concentration (MIC) assay. While
counts decreased in the wastewater with time under aerobic conditions, the prevalence of phylogroup B2 had increased to 73% on day 14. Furthermore, the MIC assay revealed that the abundance of antibiotic-resistant
also increased on day 14. After batch-mixing the experiments under aerobic conditions, the surviving antibiotic-resistant
included mainly multidrug-resistant and beta-lactamase-producing isolates belonging to phylogroup B2. These results suggest that the phylogroup B2 isolates that have acquired antibiotic resistance had a high survivability in the treated wastewater.</description><subject>Aerobic conditions</subject><subject>Antibiotic resistance</subject><subject>antibiotic resistant</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Minimum inhibitory concentration</subject><subject>Multidrug resistance</subject><subject>Municipal wastewater</subject><subject>Phylogeny</subject><subject>phylogroups</subject><subject>Staphylococcus infections</subject><subject>Survivability</subject><subject>Wastewater</subject><subject>Wastewater treatment</subject><subject>Water treatment</subject><subject>β Lactamase</subject><issn>2079-6382</issn><issn>2079-6382</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptkm1rFDEQxxdRbKn9BIIEfOObrXnYZJM3wvWoWqhYfMCXIcnO7uXYS85kt9Lv4IduzqtHKyaQGTL_-U0yTFW9JPiMMYXfmjB56-PkXSYE091-Uh1T3KpaMEmfPvCPqtOc17gsRZjE8nl1xDjFxeXH1e9rSNnnCYIDFHu0OHDrL7ALlELoIrsVJO9W3iAXR4--Tsn4kNE5jDEMPgxoimhaATqn6Hp1O8YhxXmLfECf5uCd35oR_TClyi8zQUJz6Mq5gBStd2gZQ-cnH0N-UT3rzZjh9N6eVN_fX3xbfqyvPn-4XC6uascFn2pOrCUYJJFMUaBYmaaVSraWG-iU5L21BhonlOzbTjTKCdtx7hgTXPVtz9lJdbnndtGs9Tb5jUm3Ohqv_1zENGiTSgtG0ByIMY1qjbSugdYq6TBv2oYawY0wtLDe7Vnb2W6gcxBKb8ZH0MeR4Fd6iDdaSiEpYwXw5h6Q4s8Z8qQ3PjsYRxMgzllTwZhs20aKIn39j3Qd5xRKq3YqSpTgZAdke5VLMecE_eExBOvd8Oj_DE_JevXwH4ecv6PC7gDIhcWN</recordid><startdate>20220204</startdate><enddate>20220204</enddate><creator>Xie, Hui</creator><creator>Ogura, Yoshitoshi</creator><creator>Suzuki, Yoshihiro</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-3550-9870</orcidid><orcidid>https://orcid.org/0000-0002-1148-5379</orcidid></search><sort><creationdate>20220204</creationdate><title>Persistence of Antibiotic-Resistant Escherichia coli Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions</title><author>Xie, Hui ; Ogura, Yoshitoshi ; Suzuki, Yoshihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c565t-51bb10e818392e209a478987b5aed985fbbae4c698f7d649c6bd55c33659f7f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Aerobic conditions</topic><topic>Antibiotic resistance</topic><topic>antibiotic resistant</topic><topic>Antibiotics</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Drug resistance</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Minimum inhibitory concentration</topic><topic>Multidrug resistance</topic><topic>Municipal wastewater</topic><topic>Phylogeny</topic><topic>phylogroups</topic><topic>Staphylococcus infections</topic><topic>Survivability</topic><topic>Wastewater</topic><topic>Wastewater treatment</topic><topic>Water treatment</topic><topic>β Lactamase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xie, Hui</creatorcontrib><creatorcontrib>Ogura, Yoshitoshi</creatorcontrib><creatorcontrib>Suzuki, Yoshihiro</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Antibiotics (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xie, Hui</au><au>Ogura, Yoshitoshi</au><au>Suzuki, Yoshihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Persistence of Antibiotic-Resistant Escherichia coli Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions</atitle><jtitle>Antibiotics (Basel)</jtitle><addtitle>Antibiotics (Basel)</addtitle><date>2022-02-04</date><risdate>2022</risdate><volume>11</volume><issue>2</issue><spage>202</spage><pages>202-</pages><issn>2079-6382</issn><eissn>2079-6382</eissn><abstract>is classified into four major phylogenetic groups (A, B1, B2, and D) that are associated with antibiotic resistance genes. Although antibiotic-resistant
is commonly detected in municipal wastewater, little is known about the relationship between the phylogenetic groups and antibiotic-resistant
in wastewater. In this study, the survival of
in wastewater and the changes to the relationships between each phylogroup and the antibiotic-resistant profiles of
isolates from wastewater were investigated under aerobic conditions for 14 days. The isolates were classified into the phylogroups A, B1, B2, and D or others by multiplex PCR. In addition, the susceptibility of the isolates to 11 antibiotics was assessed with the minimum inhibitory concentration (MIC) assay. While
counts decreased in the wastewater with time under aerobic conditions, the prevalence of phylogroup B2 had increased to 73% on day 14. Furthermore, the MIC assay revealed that the abundance of antibiotic-resistant
also increased on day 14. After batch-mixing the experiments under aerobic conditions, the surviving antibiotic-resistant
included mainly multidrug-resistant and beta-lactamase-producing isolates belonging to phylogroup B2. These results suggest that the phylogroup B2 isolates that have acquired antibiotic resistance had a high survivability in the treated wastewater.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>35203805</pmid><doi>10.3390/antibiotics11020202</doi><orcidid>https://orcid.org/0000-0003-3550-9870</orcidid><orcidid>https://orcid.org/0000-0002-1148-5379</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Antibiotics (Basel), 2022-02, Vol.11 (2), p.202 |
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| subjects | Aerobic conditions Antibiotic resistance antibiotic resistant Antibiotics Antimicrobial agents Bacteria Drug resistance E coli Escherichia coli Minimum inhibitory concentration Multidrug resistance Municipal wastewater Phylogeny phylogroups Staphylococcus infections Survivability Wastewater Wastewater treatment Water treatment β Lactamase |
| title | Persistence of Antibiotic-Resistant Escherichia coli Strains Belonging to the B2 Phylogroup in Municipal Wastewater under Aerobic Conditions |
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