Analysis of the Tomato mTERF Gene Family and Study of the Stress Resistance Function of SLmTERF-13

• Published in: Plants (Basel) 2023-08, Vol.12 (15), p.2862
• Main Authors: Su, Ao, Ge, Siyu, Zhou, Boyan, Wang, Ziyu, Zhou, Liping, Zhang, Ziwei, Yan, Xiaoyu, Wang, Yu, Li, Dalong, Zhang, He, Xu, Xiangyang, Zhao, Tingting
• Format: Article
• Language: English
• Subjects: Abiotic stress; Amino acids; Bioinformatics; Chloroplasts; Chromosomes; Corn; DNA-binding protein; Drought; Environmental stress; Gene expression; gene family; gene silencing; Genes; Genomes; Localization; Low temperature; Mitochondria; Mitochondrial DNA; Nucleic acids; Pattern analysis; Phylogenetics; Plant growth; Proteins; Regulatory sequences; Salts; SLmTERF13; Stress analysis; stress resistance; tomato; Tomatoes; Transcription termination
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants12152862

Mitochondrial transcription termination factor (mTERF) is a DNA-binding protein that is encoded by nuclear genes, ultimately functions in mitochondria and can affect gene expression. By combining with mitochondrial nucleic acids, mTERF regulates the replication, transcription and translation of mitochondrial genes and plays an important role in the response of plants to abiotic stress. However, there are few studies on genes in tomato, which limits the in-depth study and utilization of mTERF family genes in tomato stress resistance regulation. In this study, a total of 28 gene family members were obtained through genome-wide mining and identification of the tomato gene family. Bioinformatics analysis showed that all members of the family contained environmental stress or hormone response elements. Gene expression pattern analysis showed that the selected genes had different responses to drought, high salt and low temperature stress. Most of the genes played key roles under drought and salt stress, and the response patterns were more similar. The VIGS method was used to silence the gene, which was significantly upregulated under drought and salt stress, and it was found that the resistance ability of silenced plants was decreased under both kinds of stress, indicating that the gene was involved in the regulation of the tomato abiotic stress response. These results provide important insights for further evolutionary studies and contribute to a better understanding of the role of the genes in tomato growth and development and abiotic stress response, which will ultimately play a role in future studies of tomato gene function.