Proteomic analysis and interactions network in leaves of mycorrhizal and nonmycorrhizal sorghum plants under water deficit

• Published in: PeerJ (San Francisco, CA) CA), 2020-04, Vol.8, p.e8991-e8991, Article e8991
• Main Authors: Olalde-Portugal, Víctor, Cabrera-Ponce, José Luis, Gastelum-Arellanez, Argel, Guerrero-Rangel, Armando, Winkler, Robert, Valdés-Rodríguez, Silvia
• Format: Article
• Language: English
• Subjects: 3-Mercaptopyruvate sulfurtransferase; Abiotic stress; Agricultural Science; Arbuscular mycorrhizal fungi; Arbuscular mycorrhizas; ATP synthase; Biochemistry; Bioinformatics; Biotechnology; Carbon; Drought; Drought resistance; Energy metabolism; Fungi; Gene expression; Gene network; Guanine; High-performance liquid chromatography; Leaves; Malate dehydrogenase; Metabolic pathways; Metabolism; Mitochondria; Molecular Biology; Nitrogen; Oxidative phosphorylation; Peptidase; Phosphorylation; Photosynthesis; Physiology; Plant growth; Plant Science; Protein biosynthesis; Protein folding; Protein interaction; Proteins; Protein–protein interaction; Rain; Rainforests; Ribosomal protein L1; Ribosomal protein S10; Ribosomal proteins; Seeds; Signal transduction; Sorghum; Sucrose; Variance analysis; Water deficit
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.8991

For understanding the water deficit stress mechanism in sorghum, we conducted a physiological and proteomic analysis in the leaves of L. Moench (a drought tolerant crop model) of non-colonized and colonized plants with a consortium of arbuscular mycorrhizal fungi. Physiological results indicate that mycorrhizal fungi association enhances growth and photosynthesis in plants, under normal and water deficit conditions. 2D-electrophoresis profiles revealed 51 differentially accumulated proteins in response to water deficit, of which HPLC/MS successfully identified 49. Bioinformatics analysis of protein-protein interactions revealed the participation of different metabolic pathways in nonmycorrhizal compared to mycorrhizal sorghum plants under water deficit. In noninoculated plants, the altered proteins are related to protein synthesis and folding (50S ribosomal protein L1, 30S ribosomal protein S10, Nascent polypeptide-associated complex subunit alpha), coupled with multiple signal transduction pathways, guanine nucleotide-binding beta subunit (Rack1) and peptidyl-prolyl-cis-trans isomerase (ROC4). In contrast, in mycorrhizal plants, proteins related to energy metabolism (ATP synthase-24kDa, ATP synthase ), carbon metabolism (malate dehydrogenase, triosephosphate isomerase, sucrose-phosphatase), oxidative phosphorylation (mitochondrial-processing peptidase) and sulfur metabolism (thiosulfate/3-mercaptopyruvate sulfurtransferase) were found. Our results provide a set of proteins of different metabolic pathways involved in water deficit produced by sorghum plants alone or associated with a consortium of arbuscular mycorrhizal fungi isolated from the tropical rain forest Los Tuxtlas Veracruz, México.