PCR-RFLP method to distinguish Frankliniella occidentalis, Frankliniella intonsa, Frankliniella pallida and Frankliniella tenuicornis

Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double...

Full description

Saved in:
Bibliographic Details
Published in:Journal of plant protection research 2016-01, Vol.56 (1), p.60-66
Main Authors: Przybylska, Arnika, Fiedler, Żaneta, Obrępalska-Stęplowska, Aleksandra
Format: Article
Language:English
Subjects:
detection
Frankliniella intonsa
Frankliniella occidentalis
Frankliniella pallida
Frankliniella tenuicornis
molecular diagnostics
pest control
Thrips
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Thrips from the genus Frankliniella (Thysanoptera, Thripidae) are phytophagous on crops and wild plants. Some of them cause slight economic damage, however, others including F. occidentalis and F. intonsa are responsible for considerable losses in crop production. Moreover, they constitute a double threat for host plants by not only feeding on them but also vectoring viruses, some of which are on the quarantined list of the European Plant Protection Organization. The rapid detection and differentiation between more and less harmful Frankliniella species is, therefore, important in order to combat the pests at the time of their appearance. In this study, we have undertaken to develop a method of detecting F. occidentalis, F. intonsa, F. pallida, and F. tenuicornis. The protocol is based on PCR amplification of ITS1 rDNA fragments of these insects using universal primers pair giving products of slightly distinct length for studied insects. Restriction enzymes digestion which is easy to interpret, allows for visible differentiation of all these Frankliniella species. The method was shown to be species-specific and sensitive. Even single specimens in either the larvae or adult stage could be distinguished.
ISSN:1899-007X
1427-4345
1899-007X
DOI:10.1515/jppr-2016-0009