Whole Genome Sequencing and Root Colonization Studies Reveal Novel Insights in the Biocontrol Potential and Growth Promotion by Bacillus subtilis MBI 600 on Cucumber

spp. MBI 600 is a gram-positive bacterium and is characterized as a PGPR strain involved in plant growth promotion and control of various plant pathogens which has recently been introduced into the agricultural practice. In this study we performed a Next Generation Sequencing analysis, to analyze th...

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Bibliographic Details
Published in:Frontiers in microbiology 2021-01, Vol.11, p.600393-600393
Main Authors: Samaras, Anastasios, Nikolaidis, Marios, Antequera-Gómez, Maria Luisa, Cámara-Almirón, Jesus, Romero, Diego, Moschakis, Thomas, Amoutzias, Grigoris D, Karaoglanidis, Georgios S
Format: Article
Language:English
Subjects:
Bacillus spp
Fusarium oxysporum f.sp. radicis cucumerinum
Microbiology
plant growth Promoting bacteria
root colonization
whole genome analysis
yellow fluorescence protein-tagging
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Summary:spp. MBI 600 is a gram-positive bacterium and is characterized as a PGPR strain involved in plant growth promotion and control of various plant pathogens which has recently been introduced into the agricultural practice. In this study we performed a Next Generation Sequencing analysis, to analyze the full genome of this microorganism and to characterize it taxonomically. Results showed that MBI 600 strain was phylogenetically close to other spp. strains used as biocontrol agents and identified as . GOG analysis showed clusters contributed to secondary metabolites production such as fengycin and surfactin. In addition, various genes which annotated according to other plant-associated strains, showed that play a main role in nutrient availability from soil. The root colonization ability of MBI 600 strain was analyzed with a yellow fluorescence protein ( ) tag. Confocal laser scanning microscopy of cucumber roots treated with -tagged MBI 600 cells, revealed that the strain exhibits a strong colonization ability of cucumber roots, although it is affected significantly by the growth substrate of the roots. and experiments with MBI 600 strain and f.sp. and , showed a high control ability against these soilborne pathogens. Overall, our study demonstrates the effectiveness of MBI 600 in plant growth promotion and antagonism against different pathogens, highlighting the use of this microorganism as a biocontrol agent.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2020.600393