Draft genome sequences of Mycolicibacterium peregrinum isolated from a pig with lymphadenitis and from soil on the same Japanese pig farm

• Published in: BMC research notes 2019-06, Vol.12 (1), p.341-341, Article 341
• Main Authors: Komatsu, Tetsuya, Ohya, Kenji, Sawai, Kotaro, Odoi, Justice Opare, Otsu, Keiko, Ota, Atsushi, Ito, Toshihiro, Kawai, Mikihiko, Maruyama, Fumito
• Format: Article
• Language: English
• Subjects: Analysis; Animals; Antibiotics; Beef cattle; Causes of; Data Note; Deoxyribonucleic acid; DNA; DNA sequencing; Draft genome sequence; Drug resistance; Drug Resistance, Multiple, Bacterial - genetics; Farms; Future predictions; Genes; Genetic aspects; Genome, Bacterial - genetics; Genomes; Genomics; Humans; Hybridization; Identification and classification; In silico DNA–DNA hybridization; Infection; Japan; Lymph nodes; Lymphadenitis; Lymphadenitis - microbiology; Lymphadenitis - veterinary; Lymphatic diseases; Medical schools; Microbial drug resistance; Microbial Sensitivity Tests; Mycobacteria; Mycobacteriaceae - genetics; Mycobacteriaceae - isolation & purification; Mycobacteriaceae - pathogenicity; Mycobacterium; Mycolicibacterium peregrinum; Nucleotide sequence; Pork industry; Sequence Analysis, DNA; Soil Microbiology; Swine; Swine diseases; Swine Diseases - microbiology; Taxonomy; Tetracyclines; Virulence; Virulence (Microbiology); Virulence - genetics
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-019-4380-3

Mycolicibacterium peregrinum, a rapidly growing mycobacterial species, can opportunistically infect humans and other animals. Although M. peregrinum infections in animals have been reported, the infection sources are unknown, as is information on its virulence and drug resistant genes, which limits our current understanding of this bacterium. To address this knowledge gap, we obtained draft genome sequences for two M. peregrinum isolates; one from a case of pig lymphadenitis and one from the pig farm's soil. We report here the draft genome sequences of M. peregrinum isolates 131_1 and 138 (6,451,733-bp and 6,479,047-bp). They were isolated from a pig with mesenteric lymph node lymphadenitis and from soil on the Japanese farm where the pig was reared. A sequence alignment identity score of 100% was obtained by in silico DNA-DNA hybridization of the two isolates, while 98.28% (isolate 131_1) and 98.27% (isolate 138) scores were recorded for hybridization with a human isolate. Both isolates carry arr-1, AAC(2')-Ib, RbpA, mtrA and tap drug-resistance genes. Isolates 131_1 and 138 carry 234 and 236 putative virulence genes, respectively. Therefore, environment M. peregrinum is potentially drug resistant and can cause swine lymphadenitis. Our data provides valuable new information for future studies on nontuberculous mycobacteria.