Effects of hyperthermia on the effective concentration of rocuronium and sugammadex-mediated reversal in isolated phrenic nerve hemidiaphragm preparations of rats

• Published in: BMC anesthesiology 2020-08, Vol.20 (1), p.194-194, Article 194
• Main Authors: Kim, Jin Sun, Kim, Young Mu, Kim, Ha Jung, Choi, Jae Moon, Kim, Yong Beom, Song, Jae Seok, Yang, Hong Seuk
• Format: Article
• Language: English
• Subjects: Anesthesia; Antagonist; Body temperature; Drug dosages; Ethics; Fever; Hyperthermia; Laboratory animals; Neuromuscular blockade; Neuromuscular blocking agents; Phrenic nerve; Rocuronium; Sugammadex
• ISSN: 1471-2253; 1471-2253
• DOI: 10.1186/s12871-020-01114-7

Hyperthermia is relatively rare during general anesthesia; however, a few studies have been conducted on hyperthermia and the neuromuscular blockade (NMB) induced by rocuronium, and the reversal of NMB by sugammadex. We investigated the effect of hyperthermia status on the NMB induced by rocuronium, and its reversal by sugammadex, in isolated phrenic nerve hemidiaphragm (PNHD) preparations of the rat. Thirty-three male Sprague-Dawley rat PNHD preparations were randomly assigned to three groups at different temperatures (36 °C, 38 °C, and 40 °C; each group, n = 11, in Krebs solution). The train-of-four (TOF) and twitch height responses were checked mechanomyographically. The PNHD were treated with progressively increasing doses of rocuronium and three effective concentrations (ECs), EC50, EC90, and EC95, of rocuronium were analyzed in each group via nonlinear regression analysis. Then, sugammadex was administered in doses equimolar to rocuronium. Thereafter, the T1 height (%), TOFR (%) and the duration index were measured. The EC of rocuronium (EC50, EC90, and EC95) decreased significantly in accordance with increasing temperature. The groups at 36 °C and 40 °C showed clear differences in all areas (all P