Genomic sequencing and analysis of a Chinese hamster ovary cell line using Illumina sequencing technology

Chinese hamster ovary (CHO) cells are among the most widely used hosts for therapeutic protein production. Yet few genomic resources are available to aid in engineering high-producing cell lines. High-throughput Illumina sequencing was used to generate a 1x genomic coverage of an engineered CHO cell...

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Bibliographic Details
Published in:BMC genomics 2011-01, Vol.12 (1), p.67-67, Article 67
Main Authors: Hammond, Stephanie, Swanberg, Jeffrey C, Kaplarevic, Mihailo, Lee, Kelvin H
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Alkaline Phosphatase - genetics
Animals
Artificial chromosomes
CHO Cells - metabolism
Computational Biology
Cricetinae
Cricetulus
DNA sequencing
Genetic aspects
Genetics
Genomes
Genomics
Mice
Molecular Sequence Data
Nucleotide sequencing
Ovaries
Physiological aspects
Rats
Rodents
Sequence Analysis, DNA - methods
Technological change
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Summary:Chinese hamster ovary (CHO) cells are among the most widely used hosts for therapeutic protein production. Yet few genomic resources are available to aid in engineering high-producing cell lines. High-throughput Illumina sequencing was used to generate a 1x genomic coverage of an engineered CHO cell line expressing secreted alkaline phosphatase (SEAP). Reference-guided alignment and assembly produced 3.57 million contigs and CHO-specific sequence information for ~ 18,000 mouse and ~ 19,000 rat orthologous genes. The majority of these genes are involved in metabolic processes, cellular signaling, and transport and represent attractive targets for cell line engineering. This demonstrates the applicability of next-generation sequencing technology and comparative genomic analysis in the development of CHO genomic resources.
ISSN:1471-2164
1471-2164
DOI:10.1186/1471-2164-12-67