Establishment of porcine and monkey colonic organoids for drug toxicity study

Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity, but high cost limits their uses. Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions. However, colonic organoi...

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Bibliographic Details
Published in:Cell regeneration 2021-10, Vol.10 (1), p.1-32, Article 32
Main Authors: Li, Haonan, Wang, Yalong, Zhang, Mengxian, Wang, Hong, Cui, Along, Zhao, Jianguo, Ji, Weizhi, Chen, Ye-Guang
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Cancer
Cell Biology
Cell culture
Cell differentiation
Colon
Colonic organoids
Culture
Drug development
Drug toxicity
Drug withdrawal
Gastrointestinal diseases
Intestine
Investigations
Life Sciences
Metabolism
Methodology
Monkey
Organoids
Organoids as models for development (or cell fate determination) and diseases
Pig
Prostaglandin E2
Regenerative medicine
Stem Cells
Toxicity
Wnt protein
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Summary:Pig and monkey are widely used models for exploration of human diseases and evaluation of drug efficiency and toxicity, but high cost limits their uses. Organoids have been shown to be promising models for drug test as they reasonably preserve tissue structure and functions. However, colonic organoids of pig and monkey are not yet established. Here, we report a culture medium to support the growth of porcine and monkey colonic organoids. Wnt signaling and PGE2 are important for long-term expansion of the organoids, and their withdrawal results in lineage differentiation to mature cells. Furthermore, we observe that porcine colonic organoids are closer to human colonic organoids in terms of drug toxicity response. Successful establishment of porcine and monkey colonic organoids would facilitate the mechanistic investigation of the homeostatic regulation of the intestine of these animals and is useful for drug development and toxicity studies.
ISSN:2045-9769
2045-9769
DOI:10.1186/s13619-021-00094-4