Dual-radionuclide in vivo imaging of micro-metastasis and lymph tract with submillimetre resolution

Multi-radionuclide in vivo imaging with submillimetre resolution can be a potent tool for biomedical research. While high-resolution radionuclide imaging faces challenges in sensitivity, multi-radionuclide imaging encounters difficulty due to radiation contamination, stemming from crosstalk between...

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Bibliographic Details
Published in:Scientific reports 2023-11, Vol.13 (1), p.19464-19464, Article 19464
Main Authors: Yagishita, Atsushi, Takeda, Shin’ichiro, Ohnuki, Kazunobu, Katsuragawa, Miho, Sampetrean, Oltea, Fujii, Hirofumi, Takahashi, Tadayuki
Format: Article
Language:English
Subjects:
631/1647/245
631/1647/245/2160
Computed tomography
Contamination
Humanities and Social Sciences
Immunofluorescence
Lymph nodes
Medical research
Metastases
Metastasis
multidisciplinary
Phytic acid
Radioisotopes
Science
Science (multidisciplinary)
Single photon emission computed tomography
Spheroids
Zeolites
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Summary:Multi-radionuclide in vivo imaging with submillimetre resolution can be a potent tool for biomedical research. While high-resolution radionuclide imaging faces challenges in sensitivity, multi-radionuclide imaging encounters difficulty due to radiation contamination, stemming from crosstalk between radionuclides and Compton scattering. Addressing these challenges simultaneously is imperative for multi-radionuclide high-resolution imaging. To tackle this, we developed a high-spatial-resolution and high-energy-resolution small animal single-photon emission computed tomography (SPECT) scanner, named CdTe-DSD SPECT-I. We first assessed the feasibility of multi-tracer SPECT imaging of submillimetre targets. Using the CdTe-DSD SPECT-I, we performed SPECT imaging of submillimetre zeolite spheres absorbed with 125 I - and subsequently imaged 125 I-accumulated spheroids of 200–400 µm in size within an hour, achieving clear and quantitative images. Furthermore, dual-radionuclide phantom imaging revealed a distinct image of the submillimetre sphere absorbed with 125 I - immersed in a 99m Tc-pertechnetate solution, and provided a fair quantification of each radionuclide. Lastly, in vivo imaging was conducted on a cancer-bearing mouse with lymph node micro-metastasis using dual-tracers. The results displayed dual-tracer images of lymph tract by 99m Tc-phytic acid and the submillimetre metastatic lesion by 125 I - , shown to align with the immunofluorescence image.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-46907-1