Detection of QTLs for Outcrossing-Related Traits in CSSL Population Derived from Primitive Japonica Accession Ludao in the Genetic Background of O. sativa spp. Japonica Restorer C-bao Using RSTEP-LRT Method

• Published in: Agronomy (Basel) 2020-01, Vol.10 (1), p.28
• Main Authors: Bux, Lal, Li, Dalu, Faheem, Muhammad, Ali, Nour, Sirohi, Muzafar Hussain, Ali, Mehtab, Kumbhar, Ali Nawaz, Eltahawy, Moaz Salah, Wu, Guocan, Liu, Erbao, Dang, Xiaojing, Hong, Delin
• Format: Article
• Language: English
• Subjects: Ability tests; Agricultural production; Alleles; Cell division; Chromosomes; Crop production; Cytoplasmic male sterility; flag leaf angle; flag leaf length; flag leaf width; Gene mapping; Genes; Genomes; Kinases; Leaf angle; Leaves; Likelihood ratio; Machine tools; Oryza sativa; panicle neck length; Phenotypic variations; Pollination; Polymorphism; Population; qtls; Quantitative trait loci; Rice; Seeds; Signal transduction
• ISSN: 2073-4395; 2073-4395
• DOI: 10.3390/agronomy10010028

The outcrossing traits in rice (Oryza sativa L.) affect the yield of hybrid seed production. Using a cytoplasmic male sterile (CMS) line with good outcrossing traits, such as short flag leaf length (FLL), narrow flag leaf width (FLW), wide flag leaf angle (FLA), and elongated panicle neck length (PNL), for hybrid rice seed production, it is possible to avoid the procedure of cutting flag leaves and make the supplementary pollination feasible by machine. In this study, a japonica restorer C-bao as the receptor parent and a primitive japonica accession Ludao as the donor parent were used to construct a chromosome segment substitution line (CSSL) population. The CSSL population was used to detect quantitative trait loci (QTLs) for the four outcrossing traits using a likelihood ratio test based on the stepwise regression (RSTEP-LRT) method. The CSSL population constructed consisted of 163 lines covering 90.7% of the donor genome. Among the seven QTLs detected in the CSSL population, four QTLs were detected in both years. qFLL-4 explained 6.70% of the two-year-averaged phenotypic variance, and the alleles from Ludao decreased FLL 5.1 cm. qFLA-1.1 and qFLA-1.2 explained 7.85% and 21.29% of the 2-year-averaged phenotypic variance respectively, and the alleles from Ludao increased FLA 17.38° and 31.50°. qPNL-8 explained 8.87% of the 2-year-averaged phenotypic variance, and the alleles from Ludao increased PNL 4.44 cm. These favorable alleles identified could be used to improve the outcrossing traits of parents for hybrid rice seed production in rice.