An Evaluation of the Accuracy of the Subtraction Method Used for Determining Platelet Counts in Advanced Platelet-Rich Fibrin and Concentrated Growth Factor Preparations

Platelet concentrates should be quality-assured of purity and identity prior to clinical use. Unlike for the liquid form of platelet-rich plasma, platelet counts cannot be directly determined in solid fibrin clots and are instead calculated by subtracting the counts in other liquid or semi-clotted f...

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Bibliographic Details
Published in:Dentistry journal 2017-01, Vol.5 (1), p.7
Main Authors: Watanabe, Taisuke, Isobe, Kazushige, Suzuki, Taiji, Kawabata, Hideo, Nakamura, Masayuki, Tsukioka, Tsuneyuki, Okudera, Toshimitsu, Okudera, Hajime, Uematsu, Kohya, Okuda, Kazuhiro, Nakata, Koh, Kawase, Tomoyuki
Format: Article
Language:English
Subjects:
Aggregates
Anticoagulants
Blood
Blood cells
Blood platelets
Centrifugal force
Centrifuging
concentrated growth factors
Counting
Electron microscopy
Erythrocytes
Fibrin
fractionation
Growth factors
Mathematical analysis
platelet-rich fibrin
Platelets
Quality assurance
regenerative therapy
Scanning electron microscopy
Subtraction
Thrombosis
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Summary:Platelet concentrates should be quality-assured of purity and identity prior to clinical use. Unlike for the liquid form of platelet-rich plasma, platelet counts cannot be directly determined in solid fibrin clots and are instead calculated by subtracting the counts in other liquid or semi-clotted fractions from those in whole blood samples. Having long suspected the validity of this method, we herein examined the possible loss of platelets in the preparation process. Blood samples collected from healthy male donors were immediately centrifuged for advanced platelet-rich fibrin (A-PRF) and concentrated growth factors (CGF) according to recommended centrifugal protocols. Blood cells in liquid and semi-clotted fractions were directly counted. Platelets aggregated on clot surfaces were observed by scanning electron microscopy. A higher centrifugal force increased the numbers of platelets and platelet aggregates in the liquid red blood cell fraction and the semi-clotted red thrombus in the presence and absence of the anticoagulant, respectively. Nevertheless, the calculated platelet counts in A-PRF/CGF preparations were much higher than expected, rendering the currently accepted subtraction method inaccurate for determining platelet counts in fibrin clots. To ensure the quality of solid types of platelet concentrates chairside in a timely manner, a simple and accurate platelet-counting method should be developed immediately.
ISSN:2304-6767
2304-6767
DOI:10.3390/dj5010007