Eucalyptus-Mediated Synthesized Silver Nanoparticles-Coated Urinary Catheter Inhibits Microbial Migration and Biofilm Formation

• Published in: Nanomaterials (Basel, Switzerland) Switzerland), 2022-11, Vol.12 (22), p.4059
• Main Authors: Lethongkam, Sakkarin, Paosen, Supakit, Bilhman, Siwaporn, Dumjun, Krittima, Wunnoo, Suttiwan, Choojit, Suntree, Siri, Ratchaneewan, Daengngam, Chalongrat, Voravuthikunchai, Supayang P, Bejrananda, Tanan
• Format: Article
• Language: English
• Subjects: Antiinfectives and antibacterials; Antimicrobial activity; Antimicrobial agents; antimicrobial coating; Atomic force microscopy; Bacteria; Bacterial infections; biofilm; Biofilms; Bladder; Catheters; Coatings; Confocal microscopy; Cytotoxicity; Drug resistance; E coli; Eucalyptus; Eucalyptus camaldulensis; foley catheter; Gram-negative bacteria; Green chemistry; Health aspects; Human performance; Intubation; Medical instruments; Medicine, Botanic; Medicine, Herbal; Microenvironments; Microorganisms; Morbidity; Nanoparticles; Pathogens; Plant extracts; Risk reduction; Scanning electron microscopy; Scanning microscopy; Silver; silver nanoparticles; Staphylococcus infections; Surface roughness; Synthesis; Urinary tract; Urinary tract diseases; urinary tract infection; Urinary tract infections; Urine; Urogenital system
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano12224059

Catheter-associated urinary tract infections (CAUTIs) are significant complications among catheterized patients, resulting in increased morbidity, mortality rates, and healthcare costs. Foley urinary catheters coated with synthesized silver nanoparticles (AgNPs) using leaf extract were developed using a green chemistry principle. In situ-deposited AgNPs with particle size ranging between 20 and 120 nm on the catheter surface were illustrated by scanning electron microscopy. Atomic force microscopy revealed the changes in surface roughness after coating with nanoparticles. The coated catheter could significantly inhibit microbial adhesion and biofilm formation performed in pooled human urine-supplemented media to mimic a microenvironment during infections ( 0.05). AgNPs-coated catheter exhibited broad-spectrum antimicrobial activity against important pathogens, causing CAUTIs with no cytotoxic effects on HeLa cells. A reduction in microbial viability in biofilms was observed under confocal laser scanning microscopy. A catheter bridge model demonstrated complete prevention of migration by the coated catheter. Significant inhibition of ascending motility of and along the AgNPs-coated catheter was demonstrated in an in vitro bladder model ( 0.05). The results suggested that the AgNPs-coated urinary catheter could be applied as an alternative strategy to minimize the risk of CAUTIs by preventing bacterial colonization and biofilm formation.