A Polyclonal Selex Aptamer Library Directly Allows Specific Labelling of the Human Gut Bacterium Blautia producta without Isolating Individual Aptamers

Recent studies have demonstrated that changes in the abundance of the intestinal bacterium Blautia producta, a potential probiotic, are closely associated with the development of various diseases such as obesity, diabetes, some neurodegenerative diseases, and certain cancers. However, there is still...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2022-09, Vol.27 (17), p.5693
Main Authors: Xing, Hu, Zhang, Yiting, Krämer, Markus, Kissmann, Ann-Kathrin, Henkel, Marius, Weil, Tanja, Knippschild, Uwe, Rosenau, Frank
Format: Article
Language:English
Subjects:
Abundance
Antimicrobial agents
aptamer
Aptamers
B. producta
Bacteria
biosensor
Biosensors
Blautia
Chemical modification
Colorectal cancer
Communication
Deoxyribonucleic acid
Diabetes mellitus
Digestive system
Disease
DNA
Evolution
Fluorescence
Fluorescence microscopy
Immunogenicity
Intestinal microflora
Medical research
Microbiomes
Microbiota
Nosocomial infections
Nucleic acids
Probiotics
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Summary:Recent studies have demonstrated that changes in the abundance of the intestinal bacterium Blautia producta, a potential probiotic, are closely associated with the development of various diseases such as obesity, diabetes, some neurodegenerative diseases, and certain cancers. However, there is still a lack of an effective method to detect the abundance of B. producta in the gut rapidly. Especially, DNA aptamers are now widely used as biometric components for medical testing due to their unique characteristics, including high chemical stability, low production cost, ease of chemical modification, low immunogenicity, and fast reproducibility. We successfully obtained a high-affinity nucleic acid aptamer library (B.p-R14) after 14 SELEX rounds, which efficiently discriminates B. producta in different analysis techniques including fluorometric suspension assays or fluorescence microscopy from other major gut bacteria in complex mixtures and even in human stool samples. These preliminary findings will be the basis towards aptamer-based biosensing applications for the fast and reliable monitoring of B. producta in the human gut microbiome.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules27175693